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Membrane-bound carbonic anhydrase CA IV in the human kidney
The distribution of membrane‐bound carbonic anhydrase, CA IV, was studied in human kidneys by an indirect immunoperoxidase method using a rabbit polyclonal antibody directed against human kidney CA IV. Clear staining of CA IV was found in the apical cell borders of some cells in the cortical and med...
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| Published in: | Acta physiologica Scandinavica 1991-02, Vol.141 (2), p.231-234 |
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| Main Authors: | , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c4691-9f7580e4aa5ee835bbcdfcb83e1c5f143dda7d408c71c0f654d9bfba9f78023e3 |
| container_end_page | 234 |
| container_issue | 2 |
| container_start_page | 231 |
| container_title | Acta physiologica Scandinavica |
| container_volume | 141 |
| creator | LÖNNERHOLM, G. WISTRAND, P. J. |
| description | The distribution of membrane‐bound carbonic anhydrase, CA IV, was studied in human kidneys by an indirect immunoperoxidase method using a rabbit polyclonal antibody directed against human kidney CA IV. Clear staining of CA IV was found in the apical cell borders of some cells in the cortical and medullary segments of the collecting ducts, presumably the A type of intercalated cells. Weak staining for CA IV was located in the interior of a number of collecting duct cells and in the basolateral regions of the proximal convoluted tubules. However, no staining was found in the brush border of the same tubules. This is a surprising finding, since evidence for carbonic anhydrase activity has been found biochemically and histochemically both in isolated brush‐border and baso‐lateral membranes. Further work is needed to clarify this matter. The endothelium of the peritubular capillaries also stained for CA IV. |
| doi_str_mv | 10.1111/j.1748-1716.1991.tb09072.x |
| format | article |
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J.</creator><creatorcontrib>LÖNNERHOLM, G. ; WISTRAND, P. J.</creatorcontrib><description>The distribution of membrane‐bound carbonic anhydrase, CA IV, was studied in human kidneys by an indirect immunoperoxidase method using a rabbit polyclonal antibody directed against human kidney CA IV. Clear staining of CA IV was found in the apical cell borders of some cells in the cortical and medullary segments of the collecting ducts, presumably the A type of intercalated cells. Weak staining for CA IV was located in the interior of a number of collecting duct cells and in the basolateral regions of the proximal convoluted tubules. However, no staining was found in the brush border of the same tubules. This is a surprising finding, since evidence for carbonic anhydrase activity has been found biochemically and histochemically both in isolated brush‐border and baso‐lateral membranes. Further work is needed to clarify this matter. The endothelium of the peritubular capillaries also stained for CA IV.</description><identifier>ISSN: 0001-6772</identifier><identifier>EISSN: 1365-201X</identifier><identifier>DOI: 10.1111/j.1748-1716.1991.tb09072.x</identifier><identifier>PMID: 1904675</identifier><identifier>CODEN: APSCAX</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biocarbonate secretion ; Biological and medical sciences ; carbobnic anhydrase ; Carbonic Anhydrases - analysis ; Cell Membrane - enzymology ; Cell Membrane - ultrastructure ; cell membranes ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunohistochemistry ; kidney ; Kidney - cytology ; Kidney - enzymology ; Kidney - ultrastructure ; Kidney Tubules, Collecting - cytology ; Kidney Tubules, Collecting - enzymology ; Kidney Tubules, Collecting - ultrastructure ; Kidney Tubules, Proximal - cytology ; Kidney Tubules, Proximal - enzymology ; Kidney Tubules, Proximal - ultrastructure ; Microvilli - enzymology ; Microvilli - ultrastructure ; plasma membranes ; renal tubular acidosis ; Vertebrates: digestive system</subject><ispartof>Acta physiologica Scandinavica, 1991-02, Vol.141 (2), p.231-234</ispartof><rights>1991 Scandinavian Physiological Society</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4691-9f7580e4aa5ee835bbcdfcb83e1c5f143dda7d408c71c0f654d9bfba9f78023e3</citedby><cites>FETCH-LOGICAL-c4691-9f7580e4aa5ee835bbcdfcb83e1c5f143dda7d408c71c0f654d9bfba9f78023e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1748-1716.1991.tb09072.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1748-1716.1991.tb09072.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1410,27901,27902,46024,46448</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19662121$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1904675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LÖNNERHOLM, G.</creatorcontrib><creatorcontrib>WISTRAND, P. J.</creatorcontrib><title>Membrane-bound carbonic anhydrase CA IV in the human kidney</title><title>Acta physiologica Scandinavica</title><addtitle>Acta Physiol Scand</addtitle><description>The distribution of membrane‐bound carbonic anhydrase, CA IV, was studied in human kidneys by an indirect immunoperoxidase method using a rabbit polyclonal antibody directed against human kidney CA IV. Clear staining of CA IV was found in the apical cell borders of some cells in the cortical and medullary segments of the collecting ducts, presumably the A type of intercalated cells. Weak staining for CA IV was located in the interior of a number of collecting duct cells and in the basolateral regions of the proximal convoluted tubules. However, no staining was found in the brush border of the same tubules. This is a surprising finding, since evidence for carbonic anhydrase activity has been found biochemically and histochemically both in isolated brush‐border and baso‐lateral membranes. Further work is needed to clarify this matter. The endothelium of the peritubular capillaries also stained for CA IV.</description><subject>Biocarbonate secretion</subject><subject>Biological and medical sciences</subject><subject>carbobnic anhydrase</subject><subject>Carbonic Anhydrases - analysis</subject><subject>Cell Membrane - enzymology</subject><subject>Cell Membrane - ultrastructure</subject><subject>cell membranes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>kidney</subject><subject>Kidney - cytology</subject><subject>Kidney - enzymology</subject><subject>Kidney - ultrastructure</subject><subject>Kidney Tubules, Collecting - cytology</subject><subject>Kidney Tubules, Collecting - enzymology</subject><subject>Kidney Tubules, Collecting - ultrastructure</subject><subject>Kidney Tubules, Proximal - cytology</subject><subject>Kidney Tubules, Proximal - enzymology</subject><subject>Kidney Tubules, Proximal - ultrastructure</subject><subject>Microvilli - enzymology</subject><subject>Microvilli - ultrastructure</subject><subject>plasma membranes</subject><subject>renal tubular acidosis</subject><subject>Vertebrates: digestive system</subject><issn>0001-6772</issn><issn>1365-201X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNqVkEtv1DAURi0EKtPCT0CKkGCX4GvHj8CiGkbQVi1QEK-d5Vc0mebR2hMx8-9xlFFhh_DGsu75Pl8dhJ4DLiCdV5sCRClzEMALqCootgZXWJBi9wAtgHKWEww_H6IFxhhyLgR5jI5j3KQnlYQcoSOocMkFW6A3H3xngu59boaxd5nVwQx9YzPdr_cu6Oiz1TK7-J41fbZd-2w9drrPbhrX-_0T9KjWbfRPD_cJ-vb-3dfVeX716exitbzKbckryKtaMIl9qTXzXlJmjHW1NZJ6sKyGkjqnhSuxtAIsrjkrXWVqo1NOYkI9PUEv597bMNyNPm5V10Tr2zatPYxRScwhxeU_QWBVyTjFCXw9gzYMMQZfq9vQdDrsFWA1KVYbNSlWk2I1KVYHxWqXws8Ov4ym8-5PdHaa5i8Ocx2tbutk1zbxL4xzAgQSdzpzv5rW7_9jA7W8Pl8SOjXkc0MTt35336DDjeKCCqZ-fDxT1yA5vvz8Vn2hvwGnbqbv</recordid><startdate>199102</startdate><enddate>199102</enddate><creator>LÖNNERHOLM, G.</creator><creator>WISTRAND, P. J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199102</creationdate><title>Membrane-bound carbonic anhydrase CA IV in the human kidney</title><author>LÖNNERHOLM, G. ; WISTRAND, P. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4691-9f7580e4aa5ee835bbcdfcb83e1c5f143dda7d408c71c0f654d9bfba9f78023e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Biocarbonate secretion</topic><topic>Biological and medical sciences</topic><topic>carbobnic anhydrase</topic><topic>Carbonic Anhydrases - analysis</topic><topic>Cell Membrane - enzymology</topic><topic>Cell Membrane - ultrastructure</topic><topic>cell membranes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>kidney</topic><topic>Kidney - cytology</topic><topic>Kidney - enzymology</topic><topic>Kidney - ultrastructure</topic><topic>Kidney Tubules, Collecting - cytology</topic><topic>Kidney Tubules, Collecting - enzymology</topic><topic>Kidney Tubules, Collecting - ultrastructure</topic><topic>Kidney Tubules, Proximal - cytology</topic><topic>Kidney Tubules, Proximal - enzymology</topic><topic>Kidney Tubules, Proximal - ultrastructure</topic><topic>Microvilli - enzymology</topic><topic>Microvilli - ultrastructure</topic><topic>plasma membranes</topic><topic>renal tubular acidosis</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LÖNNERHOLM, G.</creatorcontrib><creatorcontrib>WISTRAND, P. J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta physiologica Scandinavica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LÖNNERHOLM, G.</au><au>WISTRAND, P. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Membrane-bound carbonic anhydrase CA IV in the human kidney</atitle><jtitle>Acta physiologica Scandinavica</jtitle><addtitle>Acta Physiol Scand</addtitle><date>1991-02</date><risdate>1991</risdate><volume>141</volume><issue>2</issue><spage>231</spage><epage>234</epage><pages>231-234</pages><issn>0001-6772</issn><eissn>1365-201X</eissn><coden>APSCAX</coden><abstract>The distribution of membrane‐bound carbonic anhydrase, CA IV, was studied in human kidneys by an indirect immunoperoxidase method using a rabbit polyclonal antibody directed against human kidney CA IV. Clear staining of CA IV was found in the apical cell borders of some cells in the cortical and medullary segments of the collecting ducts, presumably the A type of intercalated cells. Weak staining for CA IV was located in the interior of a number of collecting duct cells and in the basolateral regions of the proximal convoluted tubules. However, no staining was found in the brush border of the same tubules. This is a surprising finding, since evidence for carbonic anhydrase activity has been found biochemically and histochemically both in isolated brush‐border and baso‐lateral membranes. Further work is needed to clarify this matter. The endothelium of the peritubular capillaries also stained for CA IV.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1904675</pmid><doi>10.1111/j.1748-1716.1991.tb09072.x</doi><tpages>4</tpages></addata></record> |
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| ispartof | Acta physiologica Scandinavica, 1991-02, Vol.141 (2), p.231-234 |
| issn | 0001-6772 1365-201X |
| language | eng |
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| source | Wiley Online Library Backfiles Complete |
| subjects | Biocarbonate secretion Biological and medical sciences carbobnic anhydrase Carbonic Anhydrases - analysis Cell Membrane - enzymology Cell Membrane - ultrastructure cell membranes Fundamental and applied biological sciences. Psychology Humans Immunohistochemistry kidney Kidney - cytology Kidney - enzymology Kidney - ultrastructure Kidney Tubules, Collecting - cytology Kidney Tubules, Collecting - enzymology Kidney Tubules, Collecting - ultrastructure Kidney Tubules, Proximal - cytology Kidney Tubules, Proximal - enzymology Kidney Tubules, Proximal - ultrastructure Microvilli - enzymology Microvilli - ultrastructure plasma membranes renal tubular acidosis Vertebrates: digestive system |
| title | Membrane-bound carbonic anhydrase CA IV in the human kidney |
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