in vivo Ageing of human erythrocytes and cell-surface labeling by metaperiodate and sodium borotritide

Young and old human erythrocytes, separated in vitro according to age, were labeled at the surface-sialic acid residues by sodium periodate and borotritide treatment. No qualitative difference was observed between the sialic acid derivatives of young- and old-erythrocyte membranes. The number of lab...

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Bibliographic Details
Published in:Carbohydrate research 1983-06, Vol.117, p.255-262
Main Authors: Gattegno, Liliane, Durand, Geneviève, Feger, Jeanne, Perret, Gérard, Felon, Marguerite, Cornillot, Pierre
Format: Article
Language:English
Subjects:
ABO Blood-Group System
Borohydrides - pharmacology
Erythrocyte Aging - drug effects
Erythrocyte Membrane - analysis
Erythrocytes - analysis
Humans
Membrane Proteins - blood
Periodic Acid - pharmacology
Sialic Acids - blood
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Summary:Young and old human erythrocytes, separated in vitro according to age, were labeled at the surface-sialic acid residues by sodium periodate and borotritide treatment. No qualitative difference was observed between the sialic acid derivatives of young- and old-erythrocyte membranes. The number of labeled resides was significantly decreased in the in vivo aged erythrocytes (12 ± 2.8 × 10 6; n = 8) when compared to the young ones (19.4 ± 2.8 × 10 6; n = 8). Analysis of the labeled glycoproteins by sodium dodecyl sulfate gel-electrophoresis showed that this decrease affects the PAS-1, PAS-4, PAS-2, and PAS-3 bands (glycophorins A and B) of the old-erythrocyte membranes.
ISSN:0008-6215
1873-426X
DOI:10.1016/0008-6215(83)88091-4