New use of BCG for recombinant vaccines

• Published in: Nature (London) 1991-06, Vol.351 (6326), p.456-460
• Main Authors: Stover, C. K, de la Cruz, V. F, Fuerst, T. R, Burlein, J. E, Benson, L. A, Bennett, L. T, Bansal, G. P, Young, J. F, Lee, M. H, Hatfull, G. F, Snapper, S. B, Barletta, R. G, Jacobs, W. R, Bloom, B. R
• Format: Article
• Language: English
• Subjects: AIDS/HIV; Animals; antigens; Antigens - genetics; Antigens - immunology; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Antigens, Viral - genetics; Antigens, Viral - immunology; Applied microbiology; Base Sequence; BCG; BCG Vaccine - genetics; BCG Vaccine - immunology; beta-Galactosidase - genetics; Biological and medical sciences; Cloning, Molecular; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression; genes; Genes, Bacterial; Genetic Vectors; Heat-Shock Proteins - genetics; HIV Antigens - genetics; HIV-1 - immunology; Mice; Mice, Inbred BALB C; Microbiology; Molecular Sequence Data; Mycobacterium bovis - genetics; Regulatory Sequences, Nucleic Acid; vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Synthetic - genetics; Vaccines, Synthetic - immunology
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/351456a0

BCG, a live attenuated tubercle bacillus, is the most widely used vaccine in the world and is also a useful vaccine vehicle for delivering protective antigens of multiple pathogens. Extrachromosomal and integrative expression vectors carrying the regulatory sequences for major BCG heat-shock proteins have been developed to allow expression of foreign antigens in BCG. These recombinant BCG strains can elicit long-lasting humoral and cellular immune responses to foreign antigens in mice.