Establishment of spontaneously outgrowing lymphoblastoid cell lines with Cyclosporin A

To establish spontaneously outgrowing EBV (Epstein-Barr virus) transformed lymphoid cell lines from healthy EBV-seropositive adults different culture conditions were investigated. The effect of T-cell depletion was compared with that of adding Cyclosporin A (CSA) to unseparated mononuclear blood cel...

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Bibliographic Details
Published in:Medical microbiology and immunology 1983-01, Vol.172 (2), p.87-99
Main Authors: von Knebel Doeberitz, M, Bornkamm, G W, zur Hausen, H
Format: Article
Language:English
Subjects:
Antigens, Viral - analysis
B-Lymphocytes
Cell Line
Cell Transformation, Viral
Cyclosporins - pharmacology
Epstein-Barr virus
Epstein-Barr Virus Nuclear Antigens
Herpesvirus 4, Human - immunology
Herpesvirus 4, Human - physiology
Humans
T-Lymphocytes - physiology
Virus Replication - drug effects
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Summary:To establish spontaneously outgrowing EBV (Epstein-Barr virus) transformed lymphoid cell lines from healthy EBV-seropositive adults different culture conditions were investigated. The effect of T-cell depletion was compared with that of adding Cyclosporin A (CSA) to unseparated mononuclear blood cells, either at the onset of cultivation or after 3 weeks. The highest frequency of spontaneous outgrowth of EBV-transformed cells was obtained in unseparated cultures when the addition of CSA was delayed. The presence of unimpaired T cells at the onset of the culture period appeared to have a favourable effect on the spontaneous outgrowth of EBV-carrying lymphoblastoid cell lines.
ISSN:0300-8584
1432-1831
DOI:10.1007/BF02124509