Protein composition and synthesis in the adult mouse spinal cord

Properties of spinal cord proteins were studied in adult mice subjected to unilateral crush or electrical stimulation of sciatic nerve. The protein composition of spinal tissue was determined using SDS-polyacrylamide gel electrophoresis coupled with subcellular fractionation. Comparisons of mouse sp...

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Bibliographic Details
Published in:Neurochemical research 1983-05, Vol.8 (5), p.599-619
Main Authors: Stodieck, L S, Luttges, M W
Format: Article
Language:English
Subjects:
Animals
Electric Stimulation
Kinetics
Mice
Mice, Inbred Strains
Molecular Weight
Myelin Sheath - metabolism
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - isolation & purification
Sciatic Nerve - physiology
Spinal Cord - analysis
Spinal Cord - metabolism
Sulfur Radioisotopes
Tritium
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Summary:Properties of spinal cord proteins were studied in adult mice subjected to unilateral crush or electrical stimulation of sciatic nerve. The protein composition of spinal tissue was determined using SDS-polyacrylamide gel electrophoresis coupled with subcellular fractionation. Comparisons of mouse spinal cord and brain revealed similarities in the types but differences in the concentrations of myelin associated proteins, nuclear histones and other proteins. Comparisons with sciatic nerve proteins demonstrated differences in types of proteins but similarities in the concentration of myelin proteins and nuclear histones. The short term (less than 2 hrs.) incorporation of radioactive amino acids into spinal cord proteins revealed heterogeneous rates of incorporation. Neither nerve crush six days prior to testing nor sciatic nerve stimulation had a significant effect on the protein composition or amino acid incorporation rates of spinal cord tissue. These observations suggest that known differences in spinal cord function following alterations in nerve input may be dependent upon different mechanisms than have been found in the brain.
ISSN:0364-3190
1573-6903
DOI:10.1007/BF00964700