Spermidine or spermine is essential for the aerobic growth of Saccharomyces cerevisiae

A null mutation in the SPE2 gene of Saccharomyces cerevisiae, encoding S-adenosylmethionine decarboxylase, results in cells with no detectable S-adenosylmethionine decarboxylase, spermidine, and spermine. This mutant has an absolute requirement for spermidine or spermine for growth; this requirement...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1991-07, Vol.88 (13), p.5872-5876
Main Authors: Balasundaram, D. (National Institutes of Health, Bethesda, MD), Tabor, C.W, Tabor, H
Format: Article
Language:English
Subjects:
Actins
Adenosylmethionine Decarboxylase - metabolism
Aerobiosis
AMINAS
AMINAS BIOGENICAS
AMINE
AMINE BIOGENE
Amines
Anaerobiosis
Biochemistry
Biological and medical sciences
Cell growth
Chitin
CRECIMIENTO
CROISSANCE
Diploidy
DNA Mutational Analysis
Fundamental and applied biological sciences. Psychology
Genes, Fungal
Genetic mutation
GENOTIPOS
GENOTYPE
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Haploidy
LIASAS
LYASE
MEDIO DE CULTIVO
Microbiology
MILIEU DE CULTURE
MUTACION
MUTATION
Mycology
Polyamines
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Spermidine - physiology
Spermine - physiology
Yeasts
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Description
Summary:A null mutation in the SPE2 gene of Saccharomyces cerevisiae, encoding S-adenosylmethionine decarboxylase, results in cells with no detectable S-adenosylmethionine decarboxylase, spermidine, and spermine. This mutant has an absolute requirement for spermidine or spermine for growth; this requirement is not satisfied by putrescine. Polyamine-depleted cells show a number of microscopic abnormalities that are similar to those reported for several cell division cycle (cdc) and actin mutants. These include a striking increase in cell size, a marked decrease in budding, accumulation of vesicle-like bodies, absence of specific localization of chitin-like material, and abnormal distribution of actin-like material. The absolute requirement for polyamines for growth and the microscopic abnormalities are not seen if the cultures are grown under anaerobic conditions
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.13.5872