A cytochemical study of acid phosphatase, thiamine pyrophosphatase and horseradish peroxidase in the Golgi-GERL complex of hepatoma ascites cells

Data from studies of ascitic cells of Chang hepatoma have shown that acid phosphatase (ACPase) can be localized simultaneously within the trans portion of the Golgi apparatus and in tubules of the Golgi-endoplasmic reticulum-lysosome (GERL) system. Reaction products of thiamine pyrophosphatase (TPPa...

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Bibliographic Details
Published in:Cell and tissue research 1983-01, Vol.233 (2), p.367-376
Main Authors: WANG, J. J, CHANG, J. P, HSU, C. Y, LIANG, H. M, MOLLER, P. C
Format: Article
Language:English
Subjects:
Acid Phosphatase - analysis
Animal tumors. Experimental tumors
Animals
Biological and medical sciences
Endoplasmic Reticulum - enzymology
Experimental digestive system and abdominal tumors
Golgi Apparatus - enzymology
Histocytochemistry
Horseradish Peroxidase - analysis
Liver Neoplasms, Experimental - enzymology
Liver Neoplasms, Experimental - ultrastructure
Lysosomes - enzymology
Male
Medical sciences
Microscopy, Electron
Pyrophosphatases - analysis
Rats
Rats, Inbred Strains
Thiamine Pyrophosphatase - analysis
Tumors
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Summary:Data from studies of ascitic cells of Chang hepatoma have shown that acid phosphatase (ACPase) can be localized simultaneously within the trans portion of the Golgi apparatus and in tubules of the Golgi-endoplasmic reticulum-lysosome (GERL) system. Reaction products of thiamine pyrophosphatase (TPPase) were also present consistently within trans elements of the Golgi apparatus and within GERL tubules. These new findings indicate that a close physiological association may exist between the Golgi apparatus and GERL, a concept that is consistent with previous observations of fibroblasts. When horseradish peroxidase (PO) is injected intraperitoneally into ascites-bearing rats and the ascitic cells withdrawn at different time intervals, PO could be localized within vesicles and tubules in the GERL region but could not be detected within the Golgi apparatus. Bulk-phase endocytosis requires a long time and a high concentration of PO to occur. The presence of PO within GERL indicates that this organelle may play a role in transporting or processing of certain exogenous proteins.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00238303