The expression of skin-specific gene K51 in the epidermal layer of human skin and in basal cell carcinoma cells

Gene K51 probe isolated previously from the rat genomic library has been used to study the expression of its human counterpart by in situ hybridization and Northern blot analysis. A polyA-containing transcript of human gene K51 of 3 kb size has been detected in embryonic skin. The gene is also expre...

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Bibliographic Details
Published in:Archives of Dermatological Research 1991-01, Vol.283 (2), p.113-118
Main Authors: BABAEV, V. R, BELOWA, M. E, TKACHENKO, A. V, TARARAK, E. M, KAZANTSEVA, I. A, CHUMAKOV, I. M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Northern
Carcinoma, Basal Cell - genetics
Carcinoma, Basal Cell - metabolism
Carcinoma, Basal Cell - pathology
Dermatology
Epidermal Cells
Epidermis - metabolism
Gene Expression Regulation, Neoplastic
Genes - genetics
Humans
Immunohistochemistry
Keratinocytes - metabolism
Keratins - genetics
Keratins - metabolism
Medical sciences
Skin Neoplasms - genetics
Skin Neoplasms - metabolism
Skin Neoplasms - pathology
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Summary:Gene K51 probe isolated previously from the rat genomic library has been used to study the expression of its human counterpart by in situ hybridization and Northern blot analysis. A polyA-containing transcript of human gene K51 of 3 kb size has been detected in embryonic skin. The gene is also expressed in the epidermis of newborn humans and adults, but not in the adjacent mesenchymal tissues. Immunostaining with keratin antisera revealed predominantly earlier stage expression of K51 than cytokeratin markers. Sebaceous and sweat glands also contain cells expressing K51 gene. K51 expression was found in the cells of eight individual basal cell carcinomas tested, with the level of expression lower than in keratinocytes from normal human epidermis. We propose that K51 gene expression could serve as a convenient marker for the study of the process of skin keratinocyte development and the changes in this process associated with skin cancers and dysplasia.
ISSN:0340-3696
1432-069X
DOI:10.1007/BF00371619