Isolation and identification of paralytic peptides from hemolymph of the lepidopteran insects Manduca sexta, Spodoptera exigua, and Heliothis virescens

Seven paralytic peptides were isolated and identified from lepidopteran hemolymph. All of these peptides cause rapid, rigid paralysis when injected into Manduca sexta and some other lepidopteran larvae. Each peptide contains 23 amino acid residues including 2 cysteines and the carboxyl termini are a...

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Bibliographic Details
Published in:The Journal of biological chemistry 1991-07, Vol.266 (20), p.12873-12877
Main Authors: Skinner, W.S. (Sandoz Crop Protection, Palo Alto, CA), Dennis, P.A, Li, J.P, Summerfelt, R.M, Carney, R.L, Quistad, G.B
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Chromatography, High Pressure Liquid
HELIOTHIS VIRESCENS
HEMOLINFA
Hemolymph - chemistry
HEMOLYMPHE
Larva
Lepidoptera
MANDUCA SEXTA
Molecular Sequence Data
Moths - drug effects
Moths - physiology
Noctuidae
PARALISIS
PARALYSIE
Paralysis
paralytic peptide
PEPTIDE
peptides
Peptides - chemical synthesis
Peptides - chemistry
Peptides - isolation & purification
Peptides - pharmacology
PEPTIDOS
Sequence Homology, Nucleic Acid
Sphingidae
SPODOPTERA EXIGUA
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Summary:Seven paralytic peptides were isolated and identified from lepidopteran hemolymph. All of these peptides cause rapid, rigid paralysis when injected into Manduca sexta and some other lepidopteran larvae. Each peptide contains 23 amino acid residues including 2 cysteines and the carboxyl termini are acidic. Synthetic peptides in the disulfide or reduced forms, and as carboxyl-terminal acids or amides were equally paralytic. The most potent paralytic peptide, Mas PP I, has the following sequence: H-Glu-Asn-Phe-Ala-Gly-Gly-Cys-Ala-Thr-Gly-Tyr-Leu-Arg-Thr-Ala-Asp-Gly-Arg -Cys-Lys-Pro-Thr-Phe-OH. The two peptides from M. sexta hemolymph are remarkable in that they are autoparalytic (i.e. factors in collected hemolymph that are paralytic when injected into the same larvae)
ISSN:0021-9258
1083-351X
DOI:10.1016/s0021-9258(18)98775-2