Supercritical Fluid Chromatography

Chromatographic separations with a supercritical fluid as the mobile phase were suggested more than 20 years ago. Availability of commercial hardware makes this technique more widely usable today. Many separations by this method are now carried out with supercritical carbon dioxide as the mobile pha...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1983-10, Vol.222 (4621), p.253-259
Main Author: Gere, Dennis R.
Format: Article
Language:English
Subjects:
Analytical Instruments
Average velocity
Carbon Dioxide
Chemical Phenomena
Chemistry, Physical
Chromatography
Chromatography, Liquid - methods
Flow velocity
Gas Chromatography-Mass Spectrometry - methods
Legionella - analysis
Liquids
Pressure reduction
Solutes
Solvents
Spectrophotometry, Infrared
Spectrophotometry, Ultraviolet
Supercritical fluids
Temperature
Ubiquinone - isolation & purification
Viscosity
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Description
Summary:Chromatographic separations with a supercritical fluid as the mobile phase were suggested more than 20 years ago. Availability of commercial hardware makes this technique more widely usable today. Many separations by this method are now carried out with supercritical carbon dioxide as the mobile phase and packed liquid-chromatography columns as the stationary phase. Although carbon dioxide has many practical advantages, including its near-ambient critical temperature and minimal interference with spectrometric detection, the use of other supercritical fluids or addition of modifiers to carbon dioxide may extend the applications of this technique. Some mixtures that are difficult to analyze by other chromatographic methods may be susceptible to separation by supercritical fluid chromatography. Mixtures that have been separated with supercritical carbon dioxide include resin acids with the empirical formula C$_{20}$H$_{30}$O$_{2}$ and ubiquinones from bacterial cell wall extracts of Legionella pneumophila.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.6414083