A mammalian sperm lectin related to rat hepatocyte lectin-2/3 : purification from rabbit testis and identification as a zona binding protein

In rat liver the asialoglycoprotein receptor is composed of three polypeptides, RHL-1, RHL-2 and RHL-3. In rat testis and spermatozoa a galactosyl receptor (RTG-r) which is immunologically related to RHL-2/3 has been described. We now report that in addition to its presence in the rat, an antigenic...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 1991-05, Vol.103 (2), p.155-161
Main Authors: MUNIR ABDULLAH, WIDGREN, E. E, O'RAND, M. G
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Asialoglycoprotein Receptor
Biological and medical sciences
Blotting, Western
Cross Reactions
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Glycoproteins
Humans
Immunoenzyme Techniques
Lectins - analysis
Lectins - immunology
Lectins - isolation & purification
Lectins - metabolism
Liver - chemistry
Male
Mice
Proteins
Rabbits
Rats
Receptors, Cell Surface - analysis
Receptors, Cell Surface - immunology
Receptors, Cell Surface - isolation & purification
Receptors, Cell Surface - metabolism
Receptors, Immunologic - chemistry
Receptors, Immunologic - immunology
spermatozoa
Spermatozoa - chemistry
Spermatozoa - immunology
Staining and Labeling
Testis - chemistry
Testis - immunology
Zona Pellucida - metabolism
zona-binding protein
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Description
Summary:In rat liver the asialoglycoprotein receptor is composed of three polypeptides, RHL-1, RHL-2 and RHL-3. In rat testis and spermatozoa a galactosyl receptor (RTG-r) which is immunologically related to RHL-2/3 has been described. We now report that in addition to its presence in the rat, an antigenic species of 54 kDa related to RHL-2/3 is present on rabbit, human, pig and mouse spermatozoa. Purified rabbit testis galactosyl receptor (RbTG-r) consists of two major proteins of 54 and 49 kDa, while purified rabbit liver galactose lectin consists of two major proteins of 43 and 40 kDa. In an ELISA the purified rabbit testis galactosyl receptor was shown to bind biotinylated heat solubilized rabbit zonae, while the purified liver galactose lectin did not. We conclude that one of the mammalian sperm's zona binding proteins is a galactose lectin of 54 kDa related to rat liver RHL-2/3.
ISSN:0300-8177
1573-4919
DOI:10.1007/BF00227482