Evaluation of radiolabeled and colorimetric DNA probes in comparison with an antigen screening assay for the detection of Salmonella from poultry farms

A total of 48 environmental drag-swab samples from various poultry farms were tested for the presence of Salmonella spp. by culture, an enzyme-linked immunosorbent assay-based Salmonella antigen screening (SAS) assay, and two DNA probes (radiolabeled and colorimetric). The radiolabeled DNA probe was...

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Bibliographic Details
Published in:Avian diseases 1991-04, Vol.35 (2), p.397-402
Main Authors: Hasan, J.A.K. (University of Maryland, College Park, MD), Knight, I.T, Tate, C.R, Mallinson, E.T, Miller, R.G, Colwell, R.R, Joseph, S.W
Format: Article
Language:English
Subjects:
ADN
Animals
Antigens
Antigens, Bacterial - analysis
AVES DE CORRAL
COLORIMETRIA
COLORIMETRIE
Colorimetry
CULTIVO DE CELULAS
CULTURE DE CELLULE
DIAGNOSTIC
DIAGNOSTICO
DNA Probes
DNA, Bacterial - analysis
ELISA
Enzyme-Linked Immunosorbent Assay
Evaluation Studies as Topic
Food
Food safety
Genetic hybridization
Genetic screening
Nucleic Acid Hybridization
Nucleic acids
Poultry
Poultry farms
Predictive Value of Tests
Research Notes
SALMONELLA
Salmonella - genetics
Salmonella - immunology
Salmonella - isolation & purification
Sodium
TECHNIQUE DES TRACEURS
TECNICAS DE TRAZADORES
TEST ELISA
VOLAILLE
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Summary:A total of 48 environmental drag-swab samples from various poultry farms were tested for the presence of Salmonella spp. by culture, an enzyme-linked immunosorbent assay-based Salmonella antigen screening (SAS) assay, and two DNA probes (radiolabeled and colorimetric). The radiolabeled DNA probe was allowed to hybridize with culture-positive samples (n = 8) and was found to detect Salmonella spp. in all cases (100%). Both of the probes, subsequently hybridized with culture-negative samples (n = 8), were observed to yield good agreement (91%) with the culture findings. The remaining samples (n = 32) were tested by the SAS assay, and where there was no agreement between the culture and SAS, samples were further examined by the DNA probes. Results using both probes agreed with those obtained by culturing the samples but did not agree with the SAS assay result when the ratio of samples tested to samples positive (S/P) cutoff value used was 0.5
ISSN:0005-2086
1938-4351
DOI:10.2307/1591197