Signal transduction mechanism of interleukin 6 in cultured rat mesangial cells

• Published in: FEBS letters 1991-07, Vol.285 (2), p.265-267
• Main Authors: Fukunaga, Megumu, Fujiwara, Yoshihiro, Fujibayashi, Mari, Ochi, Satoshi, Yokoyama, Kenji, Ando, Akio, Hirano, Toshio, Ueda, Naohiko, Kamada, Takenobu
• Format: Article
• Language: English
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Biological and medical sciences; Calcium - metabolism; Cells, Cultured; Dinoprostone - metabolism; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glomerular Mesangium - metabolism; Immunobiology; Inositol 1,4,5-Trisphosphate - metabolism; Interleukin 6; Interleukin-6 - pharmacology; Intracellular calcium; Kinetics; Male; Mesangial cell; mesangial cells; Phosphoinositide turnover; Prostaglandin E 2; Prostaglandin E2; Rats; Rats, Inbred Strains; Recombinant Proteins - pharmacology; Regulatory factors and their cellular receptors; signal transduction; Signal Transduction - physiology; Signal transduction mechanism; Type C Phospholipases - metabolism
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(91)80813-I

Interleukin 6 (IL-6) is one of the potent autocrine growth factors for mesangial cells. We investigated the signal transduction mechanism or IL-6 in cultured rat mesangial cells. IL-6 induced a transient increase of inositol 1,4,5-trisphosphate (Ins 1,4,5-P 3) followed by a transient and sustained increase of intracellular calcium concentration, suggesting that IL-6 stimulates phosphoinositide turnover. IL-6 also stimulated prostaglandin E 2 (PGE 2) production. The IL-6-concentration dependency in PGE 2 production was similar to that in Ins 1,4,5-P 3 production. We concluded that the action of IL-6 on mesangial cells is exerted at least partially through the enhancement of phosphoinositide turnover and PGE 2 production.