Thyroid hormone effects on the State IV proton motive force in rat liver mitochondria

In order to further investigate the mechanisms regulating the control of mitochondrial respiration by thyroid hormone, the proton motive force was measured during State IV respiration in liver mitochondria isolated from euthyroid, hyperthyroid, hypothyroid and T3-treated hypothyroid rats. The proton...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 1991-04, Vol.103 (1), p.9-13
Main Authors: Horrum, M A, Tobin, R B, Ecklund, R E
Format: Article
Language:English
Subjects:
3,5,3'-triiodothyronine
Animals
Hyperthyroidism - metabolism
Hypothyroidism - metabolism
Male
Membrane Potentials - drug effects
Mitochondria, Liver - drug effects
Mitochondria, Liver - metabolism
Oxygen Consumption - drug effects
protons
Rats
Rats, Inbred Strains
Thyroid Gland - physiology
Thyroxine - blood
Triiodothyronine - blood
Triiodothyronine - pharmacology
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Summary:In order to further investigate the mechanisms regulating the control of mitochondrial respiration by thyroid hormone, the proton motive force was measured during State IV respiration in liver mitochondria isolated from euthyroid, hyperthyroid, hypothyroid and T3-treated hypothyroid rats. The proton motive force was significantly higher in the hyperthyroid group due to an increased delta pH. The proton motive force of hypothyroid mitochondria was lower than controls due to a decreased membrane potential. The proton motive force for the T3-treated hypothyroid group did not differ from the euthyroid group due to negating changes in the pH gradient and the membrane potential. The intramitochondrial volume was decreased in the hyperthyroid group and unchanged in the other groups. The results indicate that the thyroid status alters the proton motive force in State IV through individual changes in the pH and membrane potential components of the force. The component that changes in hyperthyroid mitochondria is different from that changing in hypothyroid mitochondria.
ISSN:0300-8177
1573-4919
DOI:10.1007/BF00229588