Biofilm formation by Streptococcus pneumoniae strains and effects of human serum albumin, ibuprofen, N -acetyl- l -cysteine, amoxicillin, erythromycin, and levofloxacin

Abstract Streptococcus pneumoniae ability to produce biofilms may induce persistent infections and difficulties for eradication in vivo. We investigated the ability of 11 pneumococcal strains (serotypes 3, 6B, 9V, 19F, and 23F) to form biofilms on polystyrene plates at 16 and 24 h. The extent of bio...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2010-08, Vol.67 (4), p.311-318
Main Authors: del Prado, Gema, Ruiz, Vicente, Naves, Plinio, Rodríguez-Cerrato, Violeta, Soriano, Francisco, del Carmen Ponte, María
Format: Article
Language:English
Subjects:
Acetylcysteine - pharmacology
Amoxicillin - pharmacology
Anti-Bacterial Agents - pharmacology
Biofilm
Biofilms - drug effects
Biofilms - growth & development
Erythromycin - pharmacology
Human serum albumin
Humans
Ibuprofen
Ibuprofen - pharmacology
Infectious Disease
Internal Medicine
Levofloxacin
N-acetyl- l-cysteine
Ofloxacin - pharmacology
Serum Albumin - metabolism
Streptococcus pneumoniae
Streptococcus pneumoniae - classification
Streptococcus pneumoniae - drug effects
Streptococcus pneumoniae - growth & development
Streptococcus pneumoniae - physiology
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Summary:Abstract Streptococcus pneumoniae ability to produce biofilms may induce persistent infections and difficulties for eradication in vivo. We investigated the ability of 11 pneumococcal strains (serotypes 3, 6B, 9V, 19F, and 23F) to form biofilms on polystyrene plates at 16 and 24 h. The extent of biofilm was greater at 24 h in 10 strains, being the highest magnitude for serotype 3 strains. Human serum albumin at 25 000 μg/mL and ibuprofen at 128 μg/mL significantly reduced biofilm formation in 7 and 5 strains, respectively. Amoxicillin, erythromycin, and levofloxacin at supra-MIC concentrations were very active against planktonic cells of 3 selected strains but lower on biofilm-associated organisms in 2 strains and null against the third. Although N -acetyl- l -cysteine had very little activity against both planktonic and biofilm-forming organisms, when combined with the 3 antibiotics, a slightly enhanced activity against biofilm-embedded organisms in 1 strain and combined with amoxicillin in another one was observed.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2010.03.016