Immunohistochemical evaluation of macrophage activity and its relationship with apoptotic cell death in the polar forms of leprosy

The objective of the present study was to investigate the correlation between macrophage activity and apoptosis in the polar forms of leprosy because the immunopathological phenomena involved in these forms are still poorly understood. For this purpose, 29 skin biopsy samples obtained from patients...

Full description

Saved in:
Bibliographic Details
Published in:Microbial pathogenesis 2010-10, Vol.49 (4), p.135-140
Main Authors: Quaresma, Juarez Antônio Simões, de Oliveira Lima, Luiz Wagner, Fuzii, Hellen Thais, Libonati, Rosana Maria Feio, Pagliari, Carla, Duarte, Maria Irma Seixas
Format: Article
Language:English
Subjects:
Antigens, CD - analysis
Antigens, Differentiation, Myelomonocytic - analysis
Apoptosis
Bacterial diseases
Biological and medical sciences
Biopsy
Caspase 3 - analysis
Fundamental and applied biological sciences. Psychology
Human bacterial diseases
Humans
Immunohistochemistry
Immunopathology
Infectious diseases
Leprosy
Leprosy - immunology
Leprosy - pathology
Macrophage
Macrophages - immunology
Medical sciences
Microbiology
Microscopy
Muramidase - analysis
Nitric Oxide Synthase Type II - analysis
Skin - pathology
Tropical bacterial diseases
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of the present study was to investigate the correlation between macrophage activity and apoptosis in the polar forms of leprosy because the immunopathological phenomena involved in these forms are still poorly understood. For this purpose, 29 skin biopsy samples obtained from patients with the polar forms of leprosy were analyzed. Macrophage activity and apoptosis were evaluated by immunohistochemistry using lysozyme, CD68, iNOS and caspase 3 as markers. The nonparametric Mann–Whitney test and Spearman’s linear correlation test were used for statistical analysis. The results suggest that the apoptosis rate is under the direct influence of macrophage activity in lesions of patients with the tuberculoid form. In contrast, in lepromatous lesions other factors seem to induce programmed cell death, possibly TGF-β. Further studies are necessary to identify additional factors involved in the immunopathogenesis of leprosy.
ISSN:0882-4010
1096-1208
DOI:10.1016/j.micpath.2010.05.003