Immunogenicity and thermal stability of a combined vaccine against Haemophilus influenzae type b and Neisseria meningitidis serogroup C diseases

Abstract The immunogenicity, structure and stability of a combined conjugate vaccine against Haemophilus influenzae type b and meningococcal serogroup C (Hib/MenC) were investigated. A rat model for immunogenicity showed that antibody responses to Hib and MenC in the combined vaccine were similar to...

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Bibliographic Details
Published in:Vaccine 2010-08, Vol.28 (38), p.6228-6234
Main Authors: Saydam, Manolya, Burkin, Karena, Care, Rory, Rigsby, Peter, Bolgiano, Barbara, Mawas, Fatme
Format: Article
Language:English
Subjects:
Allergy and Immunology
Analytical chemistry
Animal models
Animals
Antibodies, Bacterial - blood
Applied microbiology
Bacteriology
Biological and medical sciences
Chromatography
Confidence intervals
Enzymes
Female
Fundamental and applied biological sciences. Psychology
Haemophilus influenzae
Haemophilus Vaccines - chemistry
Haemophilus Vaccines - immunology
Hib/MenC
High temperature
Immunogenicity
Immunoglobulin G - blood
Manufacturers
Meningococcal Vaccines - immunology
Microbiology
Miscellaneous
Molecular weight
Proteins
Rats
Rats, Sprague-Dawley
Temperature
Tetanus
Tetanus Toxoid - chemistry
Tetanus Toxoid - immunology
Thermal stability
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Combined - chemistry
Vaccines, Combined - immunology
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Summary:Abstract The immunogenicity, structure and stability of a combined conjugate vaccine against Haemophilus influenzae type b and meningococcal serogroup C (Hib/MenC) were investigated. A rat model for immunogenicity showed that antibody responses to Hib and MenC in the combined vaccine were similar to or higher than those of individual conjugates given alone, or concomitantly at separate sites. At elevated temperatures, the combination vaccine was slightly more stable than a monovalent Hib-TT vaccine, with respect to molecular size, which could be attributed to differences in the formulations. Following 5 weeks incubation at 56 °C, there was some dissociation of high molecular weight conjugate without significant loss of saccharide integrity; however, this did not significantly affect the vaccine immunogenicity, demonstrating the stability of this lyophilized vaccine.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2010.07.005