Evaluation of the diagnostic value of measuring IgG, IgM, and IgA antibodies to mycobacterial A60 antigen in active tuberculosis

Abstract The purpose of the present study was to evaluate the clinical usefulness of detection of serum immunoglobulin A (IgA), IgG, and IgM antibodies raised against the mycobacterial A60 antigen for the diagnosis and discrimination of active tuberculosis (TB) from other pulmonary diseases. Three c...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2010-09, Vol.68 (1), p.55-59
Main Authors: Ben-selma, Walid, Harizi, Hedi, Marzouk, Manel, Ben Kahla, Imen, Ben Lazreg, Foued, Ferjeni, Asma, Boukadida, Jalel
Format: Article
Language:English
Subjects:
Antibodies, Bacterial - blood
Antigens, Bacterial - immunology
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin A - blood
Immunoglobulin G - blood
Immunoglobulin M - blood
Immunoglobulins
Infectious Disease
Internal Medicine
Mycobacterial A60 antigen
Mycobacterium
Mycobacterium tuberculosis - immunology
Predictive Value of Tests
Sensitivity and Specificity
Tuberculosis
Tuberculosis - diagnosis
Tuberculosis - immunology
Tuberculosis - microbiology
Tuberculosis, Pulmonary - diagnosis
Tuberculosis, Pulmonary - immunology
Tuberculosis, Pulmonary - microbiology
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Summary:Abstract The purpose of the present study was to evaluate the clinical usefulness of detection of serum immunoglobulin A (IgA), IgG, and IgM antibodies raised against the mycobacterial A60 antigen for the diagnosis and discrimination of active tuberculosis (TB) from other pulmonary diseases. Three commercially available ELISA kits (IgA, IgG, and IgM) (ANDA Biologicals, Strasbourg, France) were evaluated simultaneously in 246 serum samples from 3 groups of patients: group I, 171 patients with active TB (128 pulmonary TB and 43 extrapulmonary TB); group II, 73 patients with pulmonary non-TB diseases; and group III, 2 leprosies patients. The sensitivities of tests ranged from 31.3% (IgA) to 94% (IgG) in pulmonary TB patients and from 21% (IgA) to 84% (IgG) in extrapulmonary TB patients. The specificities of assays varied from 92% (IgG) to 96% (IgA) in the pulmonary non-TB group. Combination of IgG with IgA and/or IgM does not improve its sensitivity. Clinical use of the A60-based serodiagnostic IgG assay is of great value for the rapid diagnosis and discrimination between active TB and pulmonary non-TB diseases. Moreover, this test could be used to increase diagnostic accuracy, especially for smear-negative TB cases, which are difficult to diagnose.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2010.05.006