Biological control of Macrophomina phaseolina by chemotactic fluorescent Pseudomonas aeruginosa PN1 and its plant growth promotory activity in chir-pine

• Published in: Crop protection 2010-10, Vol.29 (10), p.1142-1147
• Main Authors: Singh, Neetu, Kumar, Sandeep, Bajpai, Vivek K., Dubey, R.C., Maheshwari, D.K., Kang, Sun Chul
• Format: Article
• Language: English
• Subjects: Antagonism; beta-glucanase; biological control agents; chitinase; culture filtrates; cyanogen; forest trees; fungal antagonists; in vitro studies; indole acetic acid; Macrophomina phaseolina; microbial growth; phosphorus; Pinus roxburghii; Plant growth promotion; plant growth-promoting rhizobacteria; plant pathogenic fungi; Pseudomonas aeruginosa; rhizosphere; siderophores; tree growth
• ISSN: 0261-2194; 1873-6904
• DOI: 10.1016/j.cropro.2010.04.008

Ten strains of Pseudomonas aeruginosa (PN1 ˜ PN10) isolated from rhizosphere of chir-pine were tested for their plant growth promontory properties and antagonistic activities against Macrophomina phaseolina in vitro and in vivo. P. aeruginosa PN1 produced siderophore, IAA, cyanogen and solubilized phosphorus, besides producing chitinase and β-1,3-glucanase. In dual culture, P. aeruginosa PN1 caused 69% colony growth inhibition. However, cell free culture filtrate also posed inhibitory effect but to a lesser extent. After 90 days, P. aeruginosa PN1 increased plant growth and biomass in pots trial containing M. phaseolina-infested soil. PN1 showed the strong chemotaxis toward root exudates resulting in effective root colonization. Moreover, increased population in rhizosphere of these bacteria was also recorded after 90 days of treatment. Thus, chemotactic fluorescent P. aeruginosa PN1 exhibited strong antagonistic property against M. phaseolina, suppressed the disease and improved plant growth of the seedlings of chir-pine proving potential biocontrol agent.