Measuring the peptides in individual organelles with mass spectrometry

New sampling protocols combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allow the assay of single dense core vesicles. Understanding the packaging of vesicles is important as vesicles are the quanta of information for intercellular communicati...

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Published in:Nature biotechnology 2000-02, Vol.18 (2), p.172-175
Main Authors: Sweedler, Jonathan V, Rubakhin, Stanislav S, Garden, Rebecca W, Fuller, Robert R
Format: Article
Language:English
Subjects:
Agriculture
Animals
Aplysia - chemistry
Aplysia californica
atrial gland
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Cytoplasmic Granules - chemistry
Desorption
Diverse techniques
Electron microscopes
Exocrine Glands - chemistry
Fundamental and applied biological sciences. Psychology
Genes
Invertebrate Hormones - analysis
Ionization
Lasers
Life Sciences
Marine
Mass spectrometry
Microscopy
Molecular and cellular biology
Organelles - chemistry
Packaging
Peptides
Peptides - analysis
Scientific imaging
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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creator Sweedler, Jonathan V
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description New sampling protocols combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allow the assay of single dense core vesicles. Understanding the packaging of vesicles is important as vesicles are the quanta of information for intercellular communication. Using vesicles from the exocrine atrial gland of Aplysia californica as the model, a wide range of bioactive peptides are detected within each vesicle. Although the expression of the egg-laying hormone gene family of type 1 atrial gland cells has been previously examined, chemical characterization of individual 1–2 μm diameter vesicles demonstrates that products from several genes are colocalized. The mass sensitivity of MALDI MS can be further improved to enable the analysis of even smaller subcellular organelles.
doi_str_mv 10.1038/72622
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identifier ISSN: 1087-0156
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subjects Agriculture
Animals
Aplysia - chemistry
Aplysia californica
atrial gland
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Cytoplasmic Granules - chemistry
Desorption
Diverse techniques
Electron microscopes
Exocrine Glands - chemistry
Fundamental and applied biological sciences. Psychology
Genes
Invertebrate Hormones - analysis
Ionization
Lasers
Life Sciences
Marine
Mass spectrometry
Microscopy
Molecular and cellular biology
Organelles - chemistry
Packaging
Peptides
Peptides - analysis
Scientific imaging
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
title Measuring the peptides in individual organelles with mass spectrometry
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