Investigation of mealybug (hemiptera: Coccoidea: Pseudococcidae) species from Turkey by RAPD

Molecular markers are commonly used for determining the relationship between insect species. In this study, we investigated the relationship of six mealybug species,Planococcus citri (Risso),Planococcus ficus (Signoret),Planococcus vovae (Nasonov),Pseudococcus longispinus (Targioni Tozzetti),Pseudoc...

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Bibliographic Details
Published in:Phytoparasitica 2007-06, Vol.35 (3), p.232-238
Main Authors: Serçe, Ç Ulubas, Kaydan, M B, Kilinçer, A N, Ertunç, F
Format: Article
Language:English
Subjects:
Coccoidea
Ficus
Genomics
Hemiptera
Phenacoccus
Planococcus
Planococcus citri
Pseudococcidae
Pseudococcus
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Summary:Molecular markers are commonly used for determining the relationship between insect species. In this study, we investigated the relationship of six mealybug species,Planococcus citri (Risso),Planococcus ficus (Signoret),Planococcus vovae (Nasonov),Pseudococcus longispinus (Targioni Tozzetti),Pseudococcus viburni (Signoret) andPhenacoccus aceris (Signoret), sampled from Turkey, using randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). Of the 50 RAPD primers tested, 18 identified a total of 256 fragments of which all were polymorphic. Sufficient discrimination betweenP. citri andP. ficus was provided by several RAPD primers. The genetic distance values calculated for each pair-wise comparison ranged from 0.156 to 0.504. Cluster analyses of RAPD data clearly separated the species into two groups.[PUBLICATION ABSTRACT]
ISSN:0334-2123
1876-7184
DOI:10.1007/BF02981155