Plectin-like proteins are present in cells ofChlamydomonas eugametos (Volvocales)

Using both monoclonal and polyclonal antibodies against mammalian plectin (multifunctional protein cross-linking cytoskeletal structures, mainly intermediate filaments, in mammalian cells), several putative isoforms of plectin-like proteins were found in protein extracts from the green algaChlamydom...

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Bibliographic Details
Published in:Folia microbiologica 2002-10, Vol.47 (5), p.535-539
Main Authors: Hendrychová, J., Vítová, M., Bišová, K., Wiche, G., Zachleder, V.
Format: Article
Language:English
Subjects:
Antibodies
Aquatic plants
Bacteria
Cell walls
Crosslinking
Cytoplasmic membranes
Cytoskeleton
Filaments
Immunoblotting
Immunofluorescence
Intermediate filaments
Isoforms
Mammalian cells
Mammals
Monoclonal antibodies
Proteins
Volvocales
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Summary:Using both monoclonal and polyclonal antibodies against mammalian plectin (multifunctional protein cross-linking cytoskeletal structures, mainly intermediate filaments, in mammalian cells), several putative isoforms of plectin-like proteins were found in protein extracts from the green algaChlamydomonas eugametos (Volvocales). Immunofluorescence and immunoblotting revealed that some of the plectin-like proteins were present in perinuclear region or localized near the cell wall, probably being attached to the cytoplasmic membrane.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02818794