Comparative genetic study of group B streptococcal strains of human and bovine origin

The presence and restriction fragment length polymorphism (RFLP) of DNA fragments hybridizing with virulence and "house keeping" gene probes were analyzed for 87 group B streptococcal (GBS) strains of human and bovine origin. Most characteristics obtained for bovine strains were similar wh...

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Bibliographic Details
Published in:Folia microbiologica 1999-08, Vol.44 (4), p.449-453
Main Authors: Dmitriev, A, Tkáciková, L, Suvorov, A, Kantíková, M, Mikula, I, Totolyan, A
Format: Article
Language:English
Subjects:
Animals
Antigens
Bacteria
Base Sequence
Cattle
DNA Primers - genetics
DNA probes
DNA, Bacterial - genetics
Fragments
Genes, Bacterial
Genetic Variation
Humans
Hybridization
Immunoglobulin A
Microbiology
Polymorphism
Polymorphism, Restriction Fragment Length
Probes
Restriction fragment length polymorphism
Species Specificity
Streptococcus
Streptococcus agalactiae - classification
Streptococcus agalactiae - genetics
Streptococcus agalactiae - pathogenicity
Virulence
Virulence - genetics
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Summary:The presence and restriction fragment length polymorphism (RFLP) of DNA fragments hybridizing with virulence and "house keeping" gene probes were analyzed for 87 group B streptococcal (GBS) strains of human and bovine origin. Most characteristics obtained for bovine strains were similar when compared with those for human strains. The most significant degree of RFLP was discovered for the sizes of HindIII fragments containing bca gene. Human GBS strains with bac gene, encoding beta antigen with IgA binding capacity, were characterized by almost identical complex hybridization patterns with multiple gene probes. At the same time bac gene was not found among bovine GBS strains. Gene scpB that encodes C5a peptidase in all human GBS strains was detected only in 9 of 39 strains of bovine origin. These two characteristics effectively distinguished bovine GBS strains from GBS strains of human origin.
ISSN:0015-5632
1874-9356
DOI:10.1007/bf02903721