Functional expression of a recombinant toxin – rPnTx2-6 – active in erectile function in rat

In the current study, the putative cDNA for PnTx2-6 toxin of the Phoneutria nigriventer spider venom was cloned and expressed as tioredoxin fusion protein in the cytoplasm of Escherichia coli. The fusion protein was purified from the bacterial extracts by combination of immobilized Ni–ion affinity a...

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Bibliographic Details
Published in:Toxicon (Oxford) 2010-12, Vol.56 (7), p.1172-1180
Main Authors: Torres, F.S., Silva, C.N., Lanza, L.F., Santos, Agenor V., Pimenta, A.M.C., De Lima, M.E., Diniz, M.R.V.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animal poisons toxicology. Antivenoms
Animals
Araneae
Biological and medical sciences
Chromatography, High Pressure Liquid
Erectile function
Escherichia coli
Escherichia coli - genetics
Injections, Subcutaneous
Male
Medical sciences
Molecular Sequence Data
Nitric oxide
Penile Erection - drug effects
Peptides - administration & dosage
Peptides - isolation & purification
Peptides - pharmacology
Phoneutria nigriventer
Priapism - chemically induced
Rats
Recombinant Fusion Proteins - administration & dosage
Recombinant Fusion Proteins - metabolism
Recombinant Fusion Proteins - pharmacology
Recombinant toxin PnTx2-6
Spider Venoms - administration & dosage
Spider Venoms - isolation & purification
Spider Venoms - pharmacology
Toxicology
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Summary:In the current study, the putative cDNA for PnTx2-6 toxin of the Phoneutria nigriventer spider venom was cloned and expressed as tioredoxin fusion protein in the cytoplasm of Escherichia coli. The fusion protein was purified from the bacterial extracts by combination of immobilized Ni–ion affinity and gel filtration chromatographies. Then, it was cleaved by enterokinase and the generated recombinant PnTx2-6 (rPnTx2-6) was further purified by reverse-phase HPLC. Likewise the native toxin purified from the spider venom, rPnTx2-6 potentiates the erectile function when injected in rats. This result indicates that the production of functional recombinant PnTx2-6 might be an alternative to provide this basic and valuable tool for study, as well as for further understanding such complex physiological system, including its correlation with the central nervous system and local tissue factors.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2010.04.010