Expression of Trichoderma reesei exo-cellobiohydrolase I in transgenic tobacco leaves and calli

Expression of Trichoderma reesei exo-cellobiohydrolase I (CBHI) gene in transgenic tobacco was under the control of CaMV 35S promoter. In transgenic leaf tissues, CBHI activity up to 66.1 micromol/h/g total protein was observed. In transgenic calli, the highest CBHI activity was 83.6 micromol h/g to...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1999, Vol.77-79 (1-3), p.689-699
Main Authors: ZIYU DAI, HOOKER, B. S, QUESENBERRY, R. D, JIANWEI GAO
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Gene expression
Genetic engineering
Genetic technics
Hypocrea jecorina
Leaves
Methods. Procedures. Technologies
Miscellaneous
Plant tissues
Proteins
Studies
Tissues
Tobacco
Transgenic animals and transgenic plants
Transgenic plants
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Summary:Expression of Trichoderma reesei exo-cellobiohydrolase I (CBHI) gene in transgenic tobacco was under the control of CaMV 35S promoter. In transgenic leaf tissues, CBHI activity up to 66.1 micromol/h/g total protein was observed. In transgenic calli, the highest CBHI activity was 83.6 micromol h/g total protein. Protein immunoblot analysis confirms the presence of CBHI enzyme in both transgenic calli and leaf tissues. CBHI expression levels accounted for about 0.11% and 0.082% of total protein in transgenic leaf tissues and calli, respectively. Furthermore, expression of CBHI gene did not affect normal growth and development of transgenic plants.
ISSN:0273-2289
1559-0291
0273-2289
DOI:10.1385/abab:79:1-3:689