Nucleotide sequence of a cDNA clone encoding chloroplast phosphoribulokinase from Arabidopsis thaliana

An Arabidopsis thaliana (C24) cDNA library constructed in Lambda ZapII was screened using a wheat phosphoribulokinase (PRKase) probe and a number of positive clones isolated. One cDNA clone, APRK1, was selected for sequence analysis. Using the plasmid rescue technique, this clone was sequenced in bo...

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Bibliographic Details
Published in:Plant molecular biology 1991-07, Vol.17 (1), p.183-184
Main Authors: Horsnell, P.R, Raines, C.A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Arabidopsis thaliana
Base Sequence
Biological and medical sciences
chloroplasts
Chloroplasts - enzymology
cloning
Cloning, Molecular
ddbj/x58149
DNA
embl/x58149
Fundamental and applied biological sciences. Psychology
genbank/x58149
genes
Genes. Genome
kinases
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
nucleotide sequences
Phosphotransferases (Alcohol Group Acceptor)
Phosphotransferases - genetics
photosynthesis
Plant Proteins - genetics
Plants - genetics
ribulose-5-phosphate kinase
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Summary:An Arabidopsis thaliana (C24) cDNA library constructed in Lambda ZapII was screened using a wheat phosphoribulokinase (PRKase) probe and a number of positive clones isolated. One cDNA clone, APRK1, was selected for sequence analysis. Using the plasmid rescue technique, this clone was sequenced in both directions using a double-stranded sequencing method. Comparison of the Arabidopsis PRKase deduced amino acid sequence with those of wheat and spinach shows that the mature proteins are about 90% identical. This clone is being using to isolate corresponding genomic sequences to study the transcriptional regulation of this gene.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00036828