Identification by Transfection of Transforming Sequences in DNA of Human T-Cell Leukemias

DNA from human T-cell leukemia cell lines was tested for focus-inducing activity on cultures of NIH 3T3 cells. Three leukemias yielded DNA active in this assay; restriction enzyme sensitivity of this activity indicated that similar, relatively large DNA sequences were involved. Southern blot analysi...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1983-11, Vol.80 (21), p.6676-6679
Main Authors: Souyri, Michèle, Fleissner, Erwin
Format: Article
Language:English
Subjects:
3T3 cells
Animals
Cell Line
Cell lines
Cell Transformation, Neoplastic
Cloning, Molecular
DNA
Humans
Leukemia
Leukemia - genetics
Mice
NIH 3T3 cells
Oncogenes
ras genes
T cell leukemia
T-Lymphocytes - physiology
Transfection
Transformation, Genetic
Transformed cell line
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Summary:DNA from human T-cell leukemia cell lines was tested for focus-inducing activity on cultures of NIH 3T3 cells. Three leukemias yielded DNA active in this assay; restriction enzyme sensitivity of this activity indicated that similar, relatively large DNA sequences were involved. Southern blot analysis revealed conserved size classes of restriction fragments containing human repetitive (Alu) sequences in serially transfected foci derived from the active DNAs. Similar blot hybridizations with a probe specific for the human N-ras oncogene detected a 9-kilobase EcoRI fragment in all cases. DNA containing this fragment from one of the leukemias, molecularly cloned in bacteriophage λ , displayed highly amplified focus-inducing activity in transfection assays. Thus, the N-ras oncogene appears to be active in these three human leukemias of T-cell origin.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.80.21.6676