Experimental model of Klebsiella infection in mice for studies on mechanisms of local immunity of the respiratory tract

An experimental model in white mice, infected with a mildly virulent strain of Klebsiella pneumoniae, was elaborated for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal space of anaesthetised animals proved to be more suitable than the commonly...

Full description

Saved in:
Bibliographic Details
Published in:Folia microbiologica 1983-01, Vol.28 (5), p.424-429
Main Authors: Libich, J, Franĕk, J, Hásková, V
Format: Article
Language:English
Subjects:
Administration, Intranasal
Animals
Bacterial Vaccines - administration & dosage
Disease Models, Animal
Immunity
Immunization - methods
Injections
Injections, Intraperitoneal
Klebsiella Infections - immunology
Klebsiella pneumoniae
Klebsiella pneumoniae - immunology
Klebsiella pneumoniae - isolation & purification
Lung - microbiology
Male
Mice
Respiratory System - immunology
Respiratory Tract Infections - immunology
Trachea
Vaccines, Attenuated - administration & dosage
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An experimental model in white mice, infected with a mildly virulent strain of Klebsiella pneumoniae, was elaborated for studies on local immunity of the respiratory tract. Instillation of klebsiella into the supralaryngeal space of anaesthetised animals proved to be more suitable than the commonly used method of intranasal infection. The strain administered by the supralaryngeal route, persisted in the lungs of most mice at approximately equal level 1 d after infection, in some animals it could be demonstrated even after 2-3 d. Using this model (based on various rates of lung clearance), one can demonstrate faster elimination of klebsiella after a local (supralaryngeal) than systemic (intraperitoneal) immunization with a heat-inactivated vaccine, prepared from a homologous strain of K. pneumoniae.
ISSN:0015-5632
DOI:10.1007/BF02879494