Intracellular assembly of human fibrinogen

Hep-G2 cells, pulse-labeled with L-[35S]methionine, incorporate radioactivity within 2 min into precursor forms of fibrinogen and into fibrinogen. Pulse-labeled intracellular fibrinogen is first composed of radioactive B beta chains, followed by nascent A alpha chains. Radioactive gamma chains accum...

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Bibliographic Details
Published in:The Journal of biological chemistry 1983-11, Vol.258 (22), p.13407-13410
Main Authors: Yu, S, Sher, B, Kudryk, B, Redman, C M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood coagulation. Blood cells
Carcinoma, Hepatocellular - genetics
Cell Line
Coagulation factors
Fibrinogen - genetics
Fibrinogen - isolation & purification
Fundamental and applied biological sciences. Psychology
Humans
Kinetics
Liver Neoplasms - genetics
Macromolecular Substances
Molecular and cellular biology
Molecular Weight
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Summary:Hep-G2 cells, pulse-labeled with L-[35S]methionine, incorporate radioactivity within 2 min into precursor forms of fibrinogen and into fibrinogen. Pulse-labeled intracellular fibrinogen is first composed of radioactive B beta chains, followed by nascent A alpha chains. Radioactive gamma chains accumulate in the cells and later contribute, via intermediate forms, to the assembly of fibrinogen. Following a pulse-chase incubation with L-[35S]methionine, the radioactive composition of newly secreted fibrinogen also reflects the fact that there is a large intracellular pool of gamma chains.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)43926-3