Mitochondrial DNA Heteroplasmy in Drosophila mauritiana

Mitochondrial DNA extracted from an isofemale strain of Drosophila mauritiana (subgroup melanogaster) appeared to be heterogeneous in size. A short genome [S; 18,500 base pairs (bp)] and a longer one (L; 19,000 bp) coexist in the preparation. The additional 500 bp have been located within the A+T-ri...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1983-11, Vol.80 (22), p.6942-6946
Main Authors: Solignac, Michel, Monnerot, Monique, Mounolou, Jean-Claude
Format: Article
Language:English
Subjects:
Animals
Base Composition
Base Sequence
DNA
DNA Restriction Enzymes
DNA, Mitochondrial - genetics
DNA, Mitochondrial - isolation & purification
Drosophila
Drosophila - genetics
Drosophila mauritiana
Drosophilidae
Eggs
Enzymes
Female animals
Genetic heterogeneity
Genomes
Genotypes
Mitochondrial DNA
Molecular Weight
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Summary:Mitochondrial DNA extracted from an isofemale strain of Drosophila mauritiana (subgroup melanogaster) appeared to be heterogeneous in size. A short genome [S; 18,500 base pairs (bp)] and a longer one (L; 19,000 bp) coexist in the preparation. The additional 500 bp have been located within the A+T-rich region. Hpa I digest patterns suggest that the S genome may carry a duplication of a 500-bp sequence including an Hpa I site and that the L genome may carry a triplication of the same sequence. At the 30th generation of the isofemale strain, 60 female genotypes were examined individually. Half of the flies were pure either for the S or the L DNA. The remaining 50% exhibited various degrees of heteroplasmy for the two DNA types. Among metazoan animals, this D. mauritiana strain offers an exceptional situation with regard to the number of individuals heterogeneous for mtDNA and the relative stability of heteroplasmy through generations.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.80.22.6942