Study of the binding of jatrophone to Escherichia coli s-ribonucleic acid

The interaction of jatrophone with sRNA with sRNA from Escherichia coli has been investigated through UV, CD, and 1H NMR measurements. The results obtained show that the interaction with jatrophone increases the stability of the polynucleotide. It appears that the optical properties of jatrophone de...

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Bibliographic Details
Published in:FEBS letters 1983-11, Vol.164 (1), p.51-56
Main Authors: D'Alagni, Maria, De Petris, Marco, Battista Marini-Bettolo, Giovanni, Temussi, Piero Andrea
Format: Article
Language:English
Subjects:
1H NMR
Antineoplastic Agents, Phytogenic - metabolism
Aqueous
Biological and medical sciences
Circular Dichroism
Diterpenes - metabolism
Escherichia coli
Escherichia coli - metabolism
Fundamental and applied biological sciences. Psychology
Interactions. Associations
Intermolecular phenomena
Jatrophone
Magnetic Resonance Spectroscopy
Molecular biophysics
Nucleic Acid Conformation
Solution
Spectrophotometry, Ultraviolet
sRNA binding
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Summary:The interaction of jatrophone with sRNA with sRNA from Escherichia coli has been investigated through UV, CD, and 1H NMR measurements. The results obtained show that the interaction with jatrophone increases the stability of the polynucleotide. It appears that the optical properties of jatrophone depend upon the jatrophone/nucleotide ratio. The observed behaviour can only be explained by the existence of different types of interaction between jatrophone and sRNA. Even for a jatrophone/nucleotide ratio as low as 0.17 the 1H NMR spectra show a multiplicity of resonances that can only be explained by the simultaneous existence of two different binding modes involving the jatrophone molecules.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(83)80017-9