The catalytic mechanism of yeast thiosulfate reductase

Thiosulfate reductase catalyzes the desulfuration of thiosulfonates while oxidizing GSH to GSSG. Kinetic studies of the enzyme-catalyzed reaction between GSH and benzenethiosulfonate have been carried out, and direct evidence for the occurrence of glutathione persulfide as an immediate product of th...

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Bibliographic Details
Published in:The Journal of biological chemistry 1983-12, Vol.258 (24), p.15037-15045
Main Authors: Chauncey, T R, Westley, J
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Glutathione - analogs & derivatives
Glutathione - metabolism
Glutathione Disulfide
Kinetics
Oxidoreductases
Oxidoreductases - metabolism
Oxidoreductases Acting on Sulfur Group Donors
Saccharomyces cerevisiae - enzymology
Spectrophotometry, Ultraviolet
Sulfates - pharmacology
Sulfites - pharmacology
Sulfurtransferases
Thiosulfonic Acids - metabolism
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Summary:Thiosulfate reductase catalyzes the desulfuration of thiosulfonates while oxidizing GSH to GSSG. Kinetic studies of the enzyme-catalyzed reaction between GSH and benzenethiosulfonate have been carried out, and direct evidence for the occurrence of glutathione persulfide as an immediate product of the reaction has been obtained. The formal mechanism of this enzymic reaction has been shown to be rapid equilibrium-ordered with GSH as the leading substrate.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)43768-9