Metabolism of mevalonic acid in cell-free homogenates of bovine retinas. Formation of novel isoprenoid acids

The metabolism of 3RS-[2RS-3H]mevalonate in the 10,000 x g supernatant fractions of homogenates of bovine retinas has been studied. Very efficient incorporation of the labeled mevalonate, approaching quantitative utilization of its 3R-isomer, into lipid products was observed. The extent of incorpora...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1983-12, Vol.258 (24), p.15062-15070
Main Authors: Fliesler, S J, Schroepfer, G J
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
animal physiology
Animals
Biological and medical sciences
Cattle
Cell-Free System
Chromatography, High Pressure Liquid
Diterpenes - biosynthesis
Fatty Acids, Unsaturated - biosynthesis
Fundamental and applied biological sciences. Psychology
human health and safety
medicine
Mevalonic Acid - metabolism
Other biological molecules
Retina - metabolism
Sterols - biosynthesis
Terpenes, steroids. Hormones
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The metabolism of 3RS-[2RS-3H]mevalonate in the 10,000 x g supernatant fractions of homogenates of bovine retinas has been studied. Very efficient incorporation of the labeled mevalonate, approaching quantitative utilization of its 3R-isomer, into lipid products was observed. The extent of incorporation into saponifiable lipids was very substantial. Analysis of the labeled saponifiable lipids indicated that these lipids were composed almost exclusively of C15 and C20 isoprenoid acids. The major C15 acids corresponded chromatographically to trans,trans-farnesoic acid and cis,cis-farnesoic acid. The major C20 isoprenoid acids corresponded chromatographically to trans,trans,-trans-geranylgeranoic acid and an unidentified C20 isoprenoid acid containing at least one cis-double bond. Analyses of the nonsaponifiable lipids indicated the presence of labeled squalene, a C30 fraction containing lanosterol and/or 24,25-dihydrolanosterol, and a C27 fraction containing cholesterol, 5 alpha-cholest-7-en-3 beta-ol, 5 alpha-cholesta-8,24-dien-3 beta-ol, 5 alpha-cholesta-7,24-dien-3 beta-ol, and cholesta-5,24-dien-3 beta-ol. Trace components corresponding chromatographically to 5 alpha-cholest-8(14)-en-3 beta-ol and cholesta-5,7-dien-3 beta-ol were also detected.
ISSN:0021-9258
1083-351X
DOI:10.1016/s0021-9258(17)43771-9