Determinants of clathrin-coated vesicle acidification

The proton-translocating ATPase of clathrin-coated vesicles of bovine brain is characterized by ATP specificity. Chloride or bromide serve as co-ions balancing electrogenic proton pumping. ATP-driven proton translocation can be observed in the absence of chloride, provided the membrane potential is...

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Bibliographic Details
Published in:The Journal of biological chemistry 1983-12, Vol.258 (24), p.14834-14838
Main Authors: Xie, X S, Stone, D K, Racker, E
Format: Article
Language:English
Subjects:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - analogs & derivatives
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - pharmacology
Adenosine Triphosphatases - metabolism
Adenosine Triphosphate - metabolism
Animals
Biological and medical sciences
Brain - ultrastructure
Cations, Divalent - pharmacology
Cattle
Cell membranes. Ionic channels. Membrane pores
Cell structures and functions
Chlorides - metabolism
Clathrin
Coated Pits, Cell-Membrane - metabolism
Endosomes - metabolism
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Protons
Substrate Specificity
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Summary:The proton-translocating ATPase of clathrin-coated vesicles of bovine brain is characterized by ATP specificity. Chloride or bromide serve as co-ions balancing electrogenic proton pumping. ATP-driven proton translocation can be observed in the absence of chloride, provided the membrane potential is collapsed by K+ moving out in the presence of valinomycin. Chloride transport can be observed independent of proton movements in the absence of ATP, provided an inside positive membrane potential is generated by K+ moving from the outside to the inside in the presence of valinomycin.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)43737-9