A study on the positive ellipticity in the circular dichroism of ribonuclease A

The small positive elliplicity near 239 nm in the CD spectrum of RNase has been investigated as a function of pH. Theoretical calculations using CD parameters representing buried or exposed tyrosine residues have been carried out. A comparison of the theoretical calculations with experimental data s...

Full description

Saved in:
Bibliographic Details
Published in:Biophysical chemistry 1983-01, Vol.18 (4), p.391-395
Main Author: Almog, Rami
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
C.D
Cattle
Circular Dichroism
Circular dichroism: Ribonuclease A
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Molecular biophysics
Pancreas - enzymology
Protein Conformation
ribonuclease (pancreatic)
Ribonucleases
Spectroscopy : techniques and spectras
Tyrosine
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The small positive elliplicity near 239 nm in the CD spectrum of RNase has been investigated as a function of pH. Theoretical calculations using CD parameters representing buried or exposed tyrosine residues have been carried out. A comparison of the theoretical calculations with experimental data suggests that the changes in the band's intensity, as a function of pH, arise mainly from electronic transitions associated with the tyrosine residues. The buried tyrosine residues are the major contributors to the ellipticity in this region at neutral pH. At higher pH contributions from exposed residurs are also observed.
ISSN:0301-4622
1873-4200
DOI:10.1016/0301-4622(83)80052-0