Influence of Powdered Coconut Water (ACP‐318®) in In Vitro Maturation of Canine Oocytes

The objective of this study was to determine the influence of powdered coconut water (ACP‐318®) diluted in high glucose (11.0 mm) TCM199 in the achievement of nuclear in vitro maturation (IVM) of canine oocytes. Cumulus oocyte complexes (COCs) (n = 632) were randomly allocated into three experimenta...

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Bibliographic Details
Published in:Reproduction in domestic animals 2010-12, Vol.45 (6), p.1042-1046
Main Authors: Silva, AEF, Cavalcante, LF, Rodrigues, BA, Rodrigues, JL
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Culture Techniques
Cocos - chemistry
Culture Media - chemistry
Dogs
Female
Fundamental and applied biological sciences. Psychology
Mammalian reproduction. General aspects
Meiosis
Oocytes - cytology
Oocytes - physiology
Recycling centers
Success
Vertebrates: reproduction
Water - chemistry
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Summary:The objective of this study was to determine the influence of powdered coconut water (ACP‐318®) diluted in high glucose (11.0 mm) TCM199 in the achievement of nuclear in vitro maturation (IVM) of canine oocytes. Cumulus oocyte complexes (COCs) (n = 632) were randomly allocated into three experimental groups named as group 1 (control group), group 2 (5% powdered coconut water) and group 3 (10% powdered coconut water). The percentage of meiotic resumption (MR) (GVBD to MII) was 39.1% (81/207), 50.2% (108/215) and 46.6% (98/210) for groups 1, 2 and 3 respectively (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/j.1439-0531.2009.01487.x