Immunodiffusion and complement fixation assays with sera from mucormycotic-infected mice

Results using homogenate antigens of Rhizomucor pusillus against immunized rabbit sera, mouse ascites fluid and sera from mucormycotic-infected mice, detected by immunodiffusion and complement fixation assays are presented. Using immunodiffusion techniques, common antigens and unique antigens from R...

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Bibliographic Details
Published in:Mycopathologia (1975) 1983-01, Vol.83 (3), p.157-160
Main Authors: WALDORF, A. R, HALDE, C, VEDROS, N. A
Format: Article
Language:English
Subjects:
Animals
Antigens, Fungal - immunology
Biological and medical sciences
Complement Fixation Tests
Experimental mycoses and models
Immunodiffusion
Infectious diseases
Medical sciences
Mice
Mucormycosis - blood
Mucormycosis - immunology
Mycoses
Rabbits
Rhizomucor pusillus
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Summary:Results using homogenate antigens of Rhizomucor pusillus against immunized rabbit sera, mouse ascites fluid and sera from mucormycotic-infected mice, detected by immunodiffusion and complement fixation assays are presented. Using immunodiffusion techniques, common antigens and unique antigens from Rh. pusillus, Rhizopus oryzae and Absidia corymbifera were observed. Complement fixation titers of greater than 256 and 128 were obtained in immunized rabbit sera and mouse ascites fluid, respectively. However, no appreciable titers of antibody were measured from mucormycotic-infected animals by either immunodiffusion or complement fixation.
ISSN:0301-486X
1573-0832
DOI:10.1007/BF00437022