Properties of Rhodopsin Dependent on Associated Phospholipid

Rhodopsin prepared from frozen bovine retinal tissue by washing the visual cell outer segment fraction with Tergitol XH and solubilizing in Triton X-100 contains approximately 91 µmoles of phospholipid per µmole of rhodopsin and shows spectral ratios ( A 278 : A 498 = 1.85; A 400 : A 498 = 0.183)...

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Bibliographic Details
Published in:The Journal of biological chemistry 1971-02, Vol.246 (4), p.881-886
Main Authors: Zorn, M, Futterman, S
Format: Article
Language:English
Subjects:
Alcohols
Amino Acids - analysis
Animals
Cattle
Phosphatidylethanolamines
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Summary:Rhodopsin prepared from frozen bovine retinal tissue by washing the visual cell outer segment fraction with Tergitol XH and solubilizing in Triton X-100 contains approximately 91 µmoles of phospholipid per µmole of rhodopsin and shows spectral ratios ( A 278 : A 498 = 1.85; A 400 : A 498 = 0.183) approaching those of the best preparations obtainable by chromatographic procedures. Removal of most of the phospholipid associated with the protein results in a decrease in the molar extinction coefficient at 498 mµ, an increase in the reactivity of sulfhydryl groups, and an inability of the visual pigment to be regenerated after bleaching. Regenerability after bleaching and the original pattern of sulfhydryl group reactivity are restored by addition of phosphatidylethanolamine to approximately 30 µmoles of phosphatidylethanolamine per µmole of rhodopsin. It is concluded that the properties of rhodopsin are sufficiently dependent upon associated phospholipid to support the view that rhodopsin is a lipoprotein.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)62407-X