Methods for the Detection of Viral Antigen and Antibody to a Feline Leukemia Virus (A Preliminary Report)

Passive hemagglutination-inhibition and micro-immunodiffusion tests were used to detect and measure feline leukemia virus (FeLV) antigens in tumor and tissue extracts of cats. Various tissues from 12 of 13 cats with spontaneous leukemia (lymphosarcoma) contained FeLV antigen; normal cat tissues did...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1970-09, Vol.45 (3), p.607-612
Main Authors: Sibal, Louis R., Fink, Mary Alexander, Plata, Ernest J., Kohler, Barbara E., Noronha, Fernando, Lee, Kyu M.
Format: Article
Language:English
Subjects:
Animals
Antibodies - analysis
Antigens - analysis
Antigens, Viral - analysis
Cat Diseases - immunology
Cats
Goats
Hemagglutination Inhibition Tests
Immunodiffusion
Leukemia - immunology
Leukemia - microbiology
Leukemia - veterinary
Lymphoma, Non-Hodgkin - immunology
Lymphoma, Non-Hodgkin - veterinary
Methods
Rabbits
Viruses, Unclassified - immunology
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Summary:Passive hemagglutination-inhibition and micro-immunodiffusion tests were used to detect and measure feline leukemia virus (FeLV) antigens in tumor and tissue extracts of cats. Various tissues from 12 of 13 cats with spontaneous leukemia (lymphosarcoma) contained FeLV antigen; normal cat tissues did not. The predominant antigen detected in these tests is an inner component of the virion. None of 20 cat serums showed antibody to this antigen. However, by hemagglutination and focus reduction tests, serums from some normal and leukemic cats showed antibodies to an outer coat antigen of the virus. The results parallel those obtained in previous studies with murine leukemia viruses.
ISSN:0027-8874
1460-2105
1460-2105
DOI:10.1093/jnci/45.3.607