Biochemical and metabolic abnormalities in normal and osteoarthritic human articular cartilage

Incorporation of radioactive precursors into macromolecules was studied with human normal and osteoarthritic articular cartilage organ culture. Analysis of the salt extracted matrix components separated by cesium chloride buoyant density gradient centrifugation showed an increase in the specific act...

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Bibliographic Details
Published in:Arthritis and rheumatism 1984-01, Vol.27 (1), p.49-57
Main Authors: Ryu, Junnosuke, Treadwell, Benjamin V., Mankin, Henry J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carbon Radioisotopes
Cartilage, Articular - metabolism
Diseases of the osteoarticular system
Female
Humans
Hyaluronic Acid - biosynthesis
Male
Medical sciences
Osteoarthritis
Osteoarthritis - metabolism
Proteoglycans - biosynthesis
Sulfur Radioisotopes
Tritium
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Summary:Incorporation of radioactive precursors into macromolecules was studied with human normal and osteoarthritic articular cartilage organ culture. Analysis of the salt extracted matrix components separated by cesium chloride buoyant density gradient centrifugation showed an increase in the specific activities of all gradient fractions prepared from the osteoarthritic cartilage. Further analysis of these fractions showed the osteoarthritic cartilage contained 5 times as much sulfate incorporated into proteoglycans, and an even greater amount of 3H‐glucosamine incorporated into material sedimenting to the middle of the gradient. Greater than half of this radioactive middle fraction appears to be hyaluronate, as judged by the position it elutes from a DEAE column and its susceptibility to hyaluronidase digestion. This study supports earlier findings showing increased rates of macromolecular synthesis in osteoarthritis, and in addition, an even greater synthetic rate for hyaluronic acid is demonstrated.
ISSN:0004-3591
1529-0131
DOI:10.1002/art.1780270109