Loading…
Clonal growth of human acute myeloid leukemia cells (ML-1 and HL-60) in serum-free agar medium
Human acute myeloid leukemia (ML-1 and HL-60) cells grew continuously in the serum-free liquid medium supplemented with human transferrin and bovine insulin. Both ML-1 and HL-60 cells formed clusters and colonies in the serum-free agar medium supplemented with bovine serum albumin, human transferrin...
Saved in:
Published in: | Cancer research (Chicago, Ill.) Ill.), 1984-02, Vol.44 (2), p.531-535 |
---|---|
Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | |
---|---|
cites | |
container_end_page | 535 |
container_issue | 2 |
container_start_page | 531 |
container_title | Cancer research (Chicago, Ill.) |
container_volume | 44 |
creator | TAKETAZU, F KUBOTA, K KAJIGAYA, S SHIONOYA, S MOTOYOSHI, K SAITO, M TAKAKU, F MIURA, Y |
description | Human acute myeloid leukemia (ML-1 and HL-60) cells grew continuously in the serum-free liquid medium supplemented with human transferrin and bovine insulin. Both ML-1 and HL-60 cells formed clusters and colonies in the serum-free agar medium supplemented with bovine serum albumin, human transferrin, cholesterol, and L-alpha-phosphatidylcholine. Medium conditioned by phytohemagglutinin-stimulated leukocytes prepared in the absence of serum had three types of colony-stimulating factors on normal human bone marrow cells. When fetal calf serum was present, medium conditioned by phytohemagglutinin-stimulated leukocytes stimulated the clonal growth of HL-60 cells at the lower concentration. However, it inhibited that of ML-1 cells. In contrast, under serum-free conditions, medium conditioned by phytohemagglutinin-stimulated leukocytes promoted the clonal growth of both ML-1 and HL-60 cells at the lower concentrations. The study using a Sephadex G-200 column revealed that, in the serum-supplemented cultures, HL-60 cells responded to one of the three colony-stimulating factors and an activity with molecular weight of around 12,000, while ML-1 cells responded only to an activity with molecular weight of around 12,000. In the serum-free cultures, both ML-1 and HL-60 cells were stimulated by activities with molecular weights of 62,000 and 54,000, respectively. These studies demonstrate that the determination of growth factors for cell lines is dependent on culture conditions, particularly on serum component; that there is a heterogeneity of ML-1 and HL-60 cells in response to the growth factors; and that there is potential importance of demonstration of heterogeneity among different cell lines in establishing requirements for different stages of differentiation. |
format | article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_80928415</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>80928415</sourcerecordid><originalsourceid>FETCH-LOGICAL-h267t-271ae08f71f471228c2b48047648ef54e227e61b788d71e26a9e09f40f5f72d33</originalsourceid><addsrcrecordid>eNo9UFFLwzAYDKLMOf0JQh5E9CGQpGmTPspQJ1R80VfLt_bLVk3amSzI_r0Vi093xx3HcUdkLvLMMK1UfkzmnHPDcqXlKTmL8WOUueD5jMyKguuRzcn70g09OLoJw_d-SwdLt8lDT6FJe6T-gG7oWuowfaLvgDboXKQ3zxUTFPqWripW8Fva9TRiSJ7ZgEhhA4F6bLvkz8mJBRfxYsIFeXu4f12uWPXy-LS8q9hWFnrPpBaA3FgtrNJCStPItTJc6UIZtLlCKTUWYq2NabVAWUCJvLSK29xq2WbZglz_9e7C8JUw7mvfxd-x0OOQYm14KY0ar1mQyymY1uPEehc6D-FQT4eM_tXkQ2zA2QB908X_WKllqWSZ_QCFeWc-</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80928415</pqid></control><display><type>article</type><title>Clonal growth of human acute myeloid leukemia cells (ML-1 and HL-60) in serum-free agar medium</title><source>EZB Electronic Journals Library</source><creator>TAKETAZU, F ; KUBOTA, K ; KAJIGAYA, S ; SHIONOYA, S ; MOTOYOSHI, K ; SAITO, M ; TAKAKU, F ; MIURA, Y</creator><creatorcontrib>TAKETAZU, F ; KUBOTA, K ; KAJIGAYA, S ; SHIONOYA, S ; MOTOYOSHI, K ; SAITO, M ; TAKAKU, F ; MIURA, Y</creatorcontrib><description>Human acute myeloid leukemia (ML-1 and HL-60) cells grew continuously in the serum-free liquid medium supplemented with human transferrin and bovine insulin. Both ML-1 and HL-60 cells formed clusters and colonies in the serum-free agar medium supplemented with bovine serum albumin, human transferrin, cholesterol, and L-alpha-phosphatidylcholine. Medium conditioned by phytohemagglutinin-stimulated leukocytes prepared in the absence of serum had three types of colony-stimulating factors on normal human bone marrow cells. When fetal calf serum was present, medium conditioned by phytohemagglutinin-stimulated leukocytes stimulated the clonal growth of HL-60 cells at the lower concentration. However, it inhibited that of ML-1 cells. In contrast, under serum-free conditions, medium conditioned by phytohemagglutinin-stimulated leukocytes promoted the clonal growth of both ML-1 and HL-60 cells at the lower concentrations. The study using a Sephadex G-200 column revealed that, in the serum-supplemented cultures, HL-60 cells responded to one of the three colony-stimulating factors and an activity with molecular weight of around 12,000, while ML-1 cells responded only to an activity with molecular weight of around 12,000. In the serum-free cultures, both ML-1 and HL-60 cells were stimulated by activities with molecular weights of 62,000 and 54,000, respectively. These studies demonstrate that the determination of growth factors for cell lines is dependent on culture conditions, particularly on serum component; that there is a heterogeneity of ML-1 and HL-60 cells in response to the growth factors; and that there is potential importance of demonstration of heterogeneity among different cell lines in establishing requirements for different stages of differentiation.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 6607105</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Agar ; Biological and medical sciences ; Cell Division ; Cell Line ; Clone Cells - pathology ; Colony-Stimulating Factors - analysis ; Culture Media ; Hematologic and hematopoietic diseases ; Humans ; Leukemia, Myeloid, Acute - pathology ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Lymphocyte Activation ; Lymphocytes - analysis ; Medical sciences ; Phytohemagglutinins - pharmacology</subject><ispartof>Cancer research (Chicago, Ill.), 1984-02, Vol.44 (2), p.531-535</ispartof><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9729429$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6607105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TAKETAZU, F</creatorcontrib><creatorcontrib>KUBOTA, K</creatorcontrib><creatorcontrib>KAJIGAYA, S</creatorcontrib><creatorcontrib>SHIONOYA, S</creatorcontrib><creatorcontrib>MOTOYOSHI, K</creatorcontrib><creatorcontrib>SAITO, M</creatorcontrib><creatorcontrib>TAKAKU, F</creatorcontrib><creatorcontrib>MIURA, Y</creatorcontrib><title>Clonal growth of human acute myeloid leukemia cells (ML-1 and HL-60) in serum-free agar medium</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>Human acute myeloid leukemia (ML-1 and HL-60) cells grew continuously in the serum-free liquid medium supplemented with human transferrin and bovine insulin. Both ML-1 and HL-60 cells formed clusters and colonies in the serum-free agar medium supplemented with bovine serum albumin, human transferrin, cholesterol, and L-alpha-phosphatidylcholine. Medium conditioned by phytohemagglutinin-stimulated leukocytes prepared in the absence of serum had three types of colony-stimulating factors on normal human bone marrow cells. When fetal calf serum was present, medium conditioned by phytohemagglutinin-stimulated leukocytes stimulated the clonal growth of HL-60 cells at the lower concentration. However, it inhibited that of ML-1 cells. In contrast, under serum-free conditions, medium conditioned by phytohemagglutinin-stimulated leukocytes promoted the clonal growth of both ML-1 and HL-60 cells at the lower concentrations. The study using a Sephadex G-200 column revealed that, in the serum-supplemented cultures, HL-60 cells responded to one of the three colony-stimulating factors and an activity with molecular weight of around 12,000, while ML-1 cells responded only to an activity with molecular weight of around 12,000. In the serum-free cultures, both ML-1 and HL-60 cells were stimulated by activities with molecular weights of 62,000 and 54,000, respectively. These studies demonstrate that the determination of growth factors for cell lines is dependent on culture conditions, particularly on serum component; that there is a heterogeneity of ML-1 and HL-60 cells in response to the growth factors; and that there is potential importance of demonstration of heterogeneity among different cell lines in establishing requirements for different stages of differentiation.</description><subject>Agar</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Clone Cells - pathology</subject><subject>Colony-Stimulating Factors - analysis</subject><subject>Culture Media</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Leukemia, Myeloid, Acute - pathology</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Lymphocyte Activation</subject><subject>Lymphocytes - analysis</subject><subject>Medical sciences</subject><subject>Phytohemagglutinins - pharmacology</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNo9UFFLwzAYDKLMOf0JQh5E9CGQpGmTPspQJ1R80VfLt_bLVk3amSzI_r0Vi093xx3HcUdkLvLMMK1UfkzmnHPDcqXlKTmL8WOUueD5jMyKguuRzcn70g09OLoJw_d-SwdLt8lDT6FJe6T-gG7oWuowfaLvgDboXKQ3zxUTFPqWripW8Fva9TRiSJ7ZgEhhA4F6bLvkz8mJBRfxYsIFeXu4f12uWPXy-LS8q9hWFnrPpBaA3FgtrNJCStPItTJc6UIZtLlCKTUWYq2NabVAWUCJvLSK29xq2WbZglz_9e7C8JUw7mvfxd-x0OOQYm14KY0ar1mQyymY1uPEehc6D-FQT4eM_tXkQ2zA2QB908X_WKllqWSZ_QCFeWc-</recordid><startdate>19840201</startdate><enddate>19840201</enddate><creator>TAKETAZU, F</creator><creator>KUBOTA, K</creator><creator>KAJIGAYA, S</creator><creator>SHIONOYA, S</creator><creator>MOTOYOSHI, K</creator><creator>SAITO, M</creator><creator>TAKAKU, F</creator><creator>MIURA, Y</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19840201</creationdate><title>Clonal growth of human acute myeloid leukemia cells (ML-1 and HL-60) in serum-free agar medium</title><author>TAKETAZU, F ; KUBOTA, K ; KAJIGAYA, S ; SHIONOYA, S ; MOTOYOSHI, K ; SAITO, M ; TAKAKU, F ; MIURA, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-271ae08f71f471228c2b48047648ef54e227e61b788d71e26a9e09f40f5f72d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Agar</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>Cell Line</topic><topic>Clone Cells - pathology</topic><topic>Colony-Stimulating Factors - analysis</topic><topic>Culture Media</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Leukemia, Myeloid, Acute - pathology</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Lymphocyte Activation</topic><topic>Lymphocytes - analysis</topic><topic>Medical sciences</topic><topic>Phytohemagglutinins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TAKETAZU, F</creatorcontrib><creatorcontrib>KUBOTA, K</creatorcontrib><creatorcontrib>KAJIGAYA, S</creatorcontrib><creatorcontrib>SHIONOYA, S</creatorcontrib><creatorcontrib>MOTOYOSHI, K</creatorcontrib><creatorcontrib>SAITO, M</creatorcontrib><creatorcontrib>TAKAKU, F</creatorcontrib><creatorcontrib>MIURA, Y</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TAKETAZU, F</au><au>KUBOTA, K</au><au>KAJIGAYA, S</au><au>SHIONOYA, S</au><au>MOTOYOSHI, K</au><au>SAITO, M</au><au>TAKAKU, F</au><au>MIURA, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clonal growth of human acute myeloid leukemia cells (ML-1 and HL-60) in serum-free agar medium</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1984-02-01</date><risdate>1984</risdate><volume>44</volume><issue>2</issue><spage>531</spage><epage>535</epage><pages>531-535</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>Human acute myeloid leukemia (ML-1 and HL-60) cells grew continuously in the serum-free liquid medium supplemented with human transferrin and bovine insulin. Both ML-1 and HL-60 cells formed clusters and colonies in the serum-free agar medium supplemented with bovine serum albumin, human transferrin, cholesterol, and L-alpha-phosphatidylcholine. Medium conditioned by phytohemagglutinin-stimulated leukocytes prepared in the absence of serum had three types of colony-stimulating factors on normal human bone marrow cells. When fetal calf serum was present, medium conditioned by phytohemagglutinin-stimulated leukocytes stimulated the clonal growth of HL-60 cells at the lower concentration. However, it inhibited that of ML-1 cells. In contrast, under serum-free conditions, medium conditioned by phytohemagglutinin-stimulated leukocytes promoted the clonal growth of both ML-1 and HL-60 cells at the lower concentrations. The study using a Sephadex G-200 column revealed that, in the serum-supplemented cultures, HL-60 cells responded to one of the three colony-stimulating factors and an activity with molecular weight of around 12,000, while ML-1 cells responded only to an activity with molecular weight of around 12,000. In the serum-free cultures, both ML-1 and HL-60 cells were stimulated by activities with molecular weights of 62,000 and 54,000, respectively. These studies demonstrate that the determination of growth factors for cell lines is dependent on culture conditions, particularly on serum component; that there is a heterogeneity of ML-1 and HL-60 cells in response to the growth factors; and that there is potential importance of demonstration of heterogeneity among different cell lines in establishing requirements for different stages of differentiation.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>6607105</pmid><tpages>5</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0008-5472 |
ispartof | Cancer research (Chicago, Ill.), 1984-02, Vol.44 (2), p.531-535 |
issn | 0008-5472 1538-7445 |
language | eng |
recordid | cdi_proquest_miscellaneous_80928415 |
source | EZB Electronic Journals Library |
subjects | Agar Biological and medical sciences Cell Division Cell Line Clone Cells - pathology Colony-Stimulating Factors - analysis Culture Media Hematologic and hematopoietic diseases Humans Leukemia, Myeloid, Acute - pathology Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Lymphocyte Activation Lymphocytes - analysis Medical sciences Phytohemagglutinins - pharmacology |
title | Clonal growth of human acute myeloid leukemia cells (ML-1 and HL-60) in serum-free agar medium |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T23%3A40%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clonal%20growth%20of%20human%20acute%20myeloid%20leukemia%20cells%20(ML-1%20and%20HL-60)%20in%20serum-free%20agar%20medium&rft.jtitle=Cancer%20research%20(Chicago,%20Ill.)&rft.au=TAKETAZU,%20F&rft.date=1984-02-01&rft.volume=44&rft.issue=2&rft.spage=531&rft.epage=535&rft.pages=531-535&rft.issn=0008-5472&rft.eissn=1538-7445&rft.coden=CNREA8&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E80928415%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-h267t-271ae08f71f471228c2b48047648ef54e227e61b788d71e26a9e09f40f5f72d33%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=80928415&rft_id=info:pmid/6607105&rfr_iscdi=true |