Mobilization of arachidonic acid between diacyl and ether phospholipids in rabbit alveolar macrophages

The metabolism of 20:4 (arachidonic acid) in alkenylacyl, alkylacyl and diacyl lipid classes in choline glycerophospholipids (CGP) and ethanolamine glycerophospholipids (EGP) in rabbit alveolar macrophages was examined. [ 3H]20:4 was very rapidly incorporated into diacyl glycerophosphocholine (GPC)....

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Bibliographic Details
Published in:FEBS letters 1984-01, Vol.165 (2), p.273-276
Main Authors: Sugiura, Takayuki, Katayama, Osamu, Fukui, Junko, Nakagawa, Yasuhito, Waku, Keizo
Format: Article
Language:English
Subjects:
18:2, linoleic acid
20:4, arachidonic acid
Alkenylacyl derivative
Alkylacyl derivative
alveoli
Analytical, structural and metabolic biochemistry
Animals
Arachidonic Acid
Arachidonic Acids - metabolism
Biological and medical sciences
BSA, bovine serum albumin
CGP, choline glycerophospholipids
EGP, ethanolamine glycerophospholipids
Ether phospholipid
Ethers - metabolism
Fundamental and applied biological sciences. Psychology
glycerophospholipids
Glycerylphosphorylcholine - metabolism
GPC, glycerophosphocholine
GPE, glycerophosphoethanolamine
Hepes, N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid
IGP
inositol glycerophospholipids
Lipids
Macrophage
macrophages
Macrophages - metabolism
membrane turnover
Other biological molecules
PAF, platelet-activing factor
Phosphatidylethanolamines - metabolism
Pulmonary Alveoli - cytology
Rabbits
Structure-Activity Relationship
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Summary:The metabolism of 20:4 (arachidonic acid) in alkenylacyl, alkylacyl and diacyl lipid classes in choline glycerophospholipids (CGP) and ethanolamine glycerophospholipids (EGP) in rabbit alveolar macrophages was examined. [ 3H]20:4 was very rapidly incorporated into diacyl glycerophosphocholine (GPC). After the removal of free 20:4, the radioactivity was gradually lost from diacyl GPC. Concomitantly, the radioactivities in alkylacyl GPC and alkenylacyl glycerophosphoethanolamine (GPE) were increased, indicating that 20:4 was mobilized from diacyl GPC to alkylacyl GPC and alkenylacyl GPE. The mobilization was considered to be a 20:4-specific event. The gradual accumulation of 20:4 in ether phospholipids leads to a high abundance of 20:4 in these lipids. These results suggest metabolic relationships between 20:4 and ether phospholipids, including platelet-activating factor (PAF).
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)80184-2