Photoaffinity labeling of acetylcholine receptor in millisecond time scale

Photoaffinity labeling of acetylcholine receptors can be performed with a time resolution allowing to discriminate reaction sites within the receptor protein in its different functional states. This is achieved by a combination of a stopped-flow apparatus with a high energy pulse laser. The photoaff...

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Bibliographic Details
Published in:FEBS letters 1984-01, Vol.166 (1), p.146-150
Main Authors: Muhn, Peter, Fahr, Alfred, Hucho, Ferdinand
Format: Article
Language:English
Subjects:
acetycholine
Acetylcholine receptor
Affinity Labels
Animals
Biological and medical sciences
Cell physiology
Fundamental and applied biological sciences. Psychology
Ion channel
Kinetics
Macromolecular Substances
Molecular and cellular biology
Neurotransmission
Photoaffinity labeling
photoaffinity labelling
Photochemistry
Protein Binding
Receptors, Cholinergic - metabolism
Torpedo
TPMP
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Summary:Photoaffinity labeling of acetylcholine receptors can be performed with a time resolution allowing to discriminate reaction sites within the receptor protein in its different functional states. This is achieved by a combination of a stopped-flow apparatus with a high energy pulse laser. The photoaffinity label used is the lipophilic cation [ 3H]TPMP + which has been shown to be a non-competitive antagonist and a specific ion channel blocker. AChR in its resting (channel closed) and active (channel open) state incorporates the label mainly into the α-polypeptide chain of the receptor. Only several hundred milliseconds after mixing AChR with agonist labeling of δ-chains becomes significant.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)80061-7