Primary structural sequence polymorphism in the human class II MHC antigen 33.1

Monoclonal antibody 33.1 defines a non-DR, class II, human major histocompatibility complex antigen, 33.1, which appears to be distinct from other class II antigens in its cellular distribution and primary structure. To characterize the structure more fully and to determine the degree of polymorphis...

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Bibliographic Details
Published in:Immunogenetics (New York) 1984, Vol.19 (1), p.27-37
Main Authors: MEI-CHANG KUO, XUAN-MAO LI, MARTI, G. E, SACHS, J. A, SOGN, J. A, COLIGAN, J. E, KINDT, T. J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens
B-Lymphocytes - immunology
Base Sequence
Biological and medical sciences
Cell Line
Cell Transformation, Neoplastic
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Genes
Genes, MHC Class II
Herpesvirus 4, Human - genetics
HLA Antigens - genetics
Humans
Major Histocompatibility Complex
Mice
Molecular immunology
Polymorphism, Genetic
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Summary:Monoclonal antibody 33.1 defines a non-DR, class II, human major histocompatibility complex antigen, 33.1, which appears to be distinct from other class II antigens in its cellular distribution and primary structure. To characterize the structure more fully and to determine the degree of polymorphism within 33.1, a comparative N-terminal sequence study has been undertaken using a series of ten B lymphoblastoid cell lines with different DR and MB types. The results confirm that both the alpha and beta chains of 33.1 are homologues of the corresponding chains of the murine I-A antigen and indicate that while 33.1 does not appear to be identical with MB, it is closely related. Sequence analyses revealed two major variants of 33.1, corresponding to cells with specificities MB1 and MB3, respectively. Within each MB type, other polymorphisms have been detected. Cells that are MB2 do not react with monoclonal antibody 33.1. Suggestive evidence is presented that monoclonal antibody 33.1 reacts predominantly with the beta chain of the antigen. The preferential expression of 33.1 on activated B cells suggests that expression of at least the 33.1 beta chain gene is greatly enhanced in the course of B-cell activation, but the specific function of 33.1 remains to be determined.
ISSN:0093-7711
1432-1211
DOI:10.1007/BF00364473