Growth of chikungunya virus in baby hamster kidney cell (BHK-21-clone 13) suspension cultures

This report describes the methods used to obtain high titers of chikungunya virus with suspension cultures of BHK-21-clone 13 cells. The cells were grown at 37 C to a cell concentration of 10(6) to 2 x 10(6) per ml. After maximum cell growth, the cells were inoculated with chikungunya virus at a mul...

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Bibliographic Details
Published in:Applied microbiology 1971-02, Vol.21 (2), p.338-341
Main Authors: Davis, J L, Hodge, H M, Campbell, Jr, W E
Format: Article
Language:English
Subjects:
Animals
Cattle
Cell Line
Chikungunya virus - growth & development
Chikungunya virus - isolation & purification
Clone Cells - growth & development
Cricetinae
Culture Media
Culture Techniques
Immune Sera
Kidney
Mice
Trypsin
Virus Cultivation
Virus Replication
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Summary:This report describes the methods used to obtain high titers of chikungunya virus with suspension cultures of BHK-21-clone 13 cells. The cells were grown at 37 C to a cell concentration of 10(6) to 2 x 10(6) per ml. After maximum cell growth, the cells were inoculated with chikungunya virus at a multiplicity of 1 to 2 50% suckling mouse intracerebral lethal doses (SMICLD(50)) per cell in the spent Eagle's minimum essential medium for suspension cultures (MEMS), or the cell cultures were centrifuged at 200 x g and resuspended in either fresh MEMS or medium 199 prior to inoculation. The medium used had no effect on virus titer. The inoculated cultures were incubated at 34 C until the cell viability dropped to 30%, which usually occurred 28 to 30 hr postinoculation. After these procedures, chikungunya virus titers of log(10) 10.3 to 11.8 SMICLD(50) per ml were obtained.
ISSN:0003-6919
DOI:10.1128/AEM.21.2.338-341.1971