Effect of chromosomal position on amplification of transfected genes in animal cells

A single protein (CAD) contains the first three enzymatic activities of de novo undine biosynthesis. The chromosomal location of CAD genes introduced into Chinese hamster ovary cells significantly affects the frequency and cytogenetic result of their amplification. The amplification frequency in one...

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Bibliographic Details
Published in:Nature (London) 1984-02, Vol.307 (5951), p.516-520
Main Authors: Wahl, Geoffrey M, Robert de Saint Vincent, Bruno, DeRose, Margaret L
Format: Article
Language:English
Subjects:
Animals
Aspartate Carbamoyltransferase
Biological and medical sciences
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Cells, Cultured
Chromosome Mapping
Cricetinae
Dihydroorotase
Fundamental and applied biological sciences. Psychology
Gene Amplification
Gene Expression Regulation
Genetic Linkage
Genic rearrangement. Recombination. Transposable element
Humanities and Social Sciences
Molecular and cellular biology
Molecular genetics
multidisciplinary
Multienzyme Complexes
Proteins - genetics
Science
Science (multidisciplinary)
Transfection
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Summary:A single protein (CAD) contains the first three enzymatic activities of de novo undine biosynthesis. The chromosomal location of CAD genes introduced into Chinese hamster ovary cells significantly affects the frequency and cytogenetic result of their amplification. The amplification frequency in one transformant is 100-fold that of the others; in another, amplification of donated genes inserted near a centromere results in chromosome instability and rearrangements.
ISSN:0028-0836
1476-4687
DOI:10.1038/307516a0