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Effects of Guanosine 3',5'-Monophosphate on the Perfused Rat Liver
The effect of exogenous guanosine 3',5'-monophosphate (cyclic GMP) and adenosine 3',5'-monophosphate (cyclic AMP) were compared in the isolated rat liver perfused with buffer containing blood cells and albumin. Cyclic GMP was â to ½ as potent as cyclic AMP in stimulating gluco...
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Published in: | The Journal of biological chemistry 1971-04, Vol.246 (8), p.2658-2664 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The effect of exogenous guanosine 3',5'-monophosphate (cyclic GMP) and adenosine 3',5'-monophosphate (cyclic AMP) were compared
in the isolated rat liver perfused with buffer containing blood cells and albumin.
Cyclic GMP was â
to ½ as potent as cyclic AMP in stimulating glucose production, net glycogenolysis, glyconeogenesis from
lactate, and release of K + . Cyclic GMP activated glycogen phosphorylase and inhibited the incorporation of 14 C from lactate into glycogen.
The effectiveness of cyclic GMP in the perfused liver was unexpected in view of its relatively weak ability to mimic cyclic
AMP in broken cell preparations. Cyclic GMP did not act by raising the tissue level of cyclic AMP nor did it promote the release
of cyclic AMP into the medium as did glucagon or epinephrine.
Cyclic GMP was observed to accumulate in the liver to a much greater extent than cyclic AMP, and this probably accounts for
its unexpected potency. The two nucleotides were removed by the liver at similar rates.
Insulin inhibited the effects of submaximal concentrations of cyclic AMP on hepatic glycogenolysis, but did not alter the
effects of cyclic GMP. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(18)62337-3 |