The Effect of Neonatal Hyperbilirubinemia on the Measurement of Plasma Creatinine

Bilirubin interferes with the measurement of plasma creatinine by the kinetic colorimetric Jaffe reaction, the method currently used by many hospital laboratories. The purpose of the present study was to evaluate the influence of unconjugated bilirubin on the measurement of plasma creatinine by the...

Full description

Saved in:
Bibliographic Details
Published in:Clinical pediatrics 1984-03, Vol.23 (3), p.154-156
Main Authors: Feld, Leonard G., Langford, Daniel J., Schwartz, George J.
Format: Article
Language:English
Subjects:
Bilirubin - blood
Biological and medical sciences
Colorimetry - methods
Creatinine - blood
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Infant, Newborn
Jaundice, Neonatal - blood
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Medical sciences
Other diseases. Semiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bilirubin interferes with the measurement of plasma creatinine by the kinetic colorimetric Jaffe reaction, the method currently used by many hospital laboratories. The purpose of the present study was to evaluate the influence of unconjugated bilirubin on the measurement of plasma creatinine by the AutoAnalyzer Jaffe end point method. This colorimetric method is slower but more precise and accurate in the range of 0.25 to 0.5 mg/dl. We found that bilirubin from jaundiced neonates and from stock solutions did not affect the determination of creatinine chro mogen in plasma or in saline, even at concentrations as high as 25 mg/dl. We conclude that the use of the Jaffe end point AutoAnalyzer method with a blank run before each sample will provide an accurate measurement of creatinine in the plasma of the neonate with unconjugated hyper bilirubinemia.
ISSN:0009-9228
1938-2707
DOI:10.1177/000992288402300304