The Study of Differentiative Potential of the Lactating Mouse Mammary Gland in Organ Culture

The organ culture of the mammary gland of lactating mice was used to examine the response of the differentiated gland to lactogenic stimuli, insulin, cortisol, and prolactin. Time course studies showed that casein synthesis in cultured tissue decreased rapidly during the first 2 d despite the presen...

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Bibliographic Details
Published in:In Vitro 1984-01, Vol.20 (1), p.59-65
Main Authors: Perry, John W., Oka, Takami
Format: Article
Language:English
Subjects:
Animal cells
Animal glands
Animals
Biological and medical sciences
Biotechnology
Caseins - biosynthesis
Cell Division
Daughter cells
DNA
Epithelial cells
Eukaryotic cell cultures
Female
Fundamental and applied biological sciences. Psychology
Hormone metabolism and regulation
Hormones
Hydrocortisone - pharmacology
Insulin
Insulin - pharmacology
Lactalbumin - biosynthesis
Lactation
Mammary glands
Mammary Glands, Animal - metabolism
Messenger RNA
Methods. Procedures. Technologies
Mice
Mice, Inbred C3H
Milk protein
Miscellaneous
Organ Culture Techniques
Pregnancy
Pregnancy. Parturition. Lactation
Prolactin - pharmacology
RNA, Messenger - metabolism
Vertebrates: reproduction
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Summary:The organ culture of the mammary gland of lactating mice was used to examine the response of the differentiated gland to lactogenic stimuli, insulin, cortisol, and prolactin. Time course studies showed that casein synthesis in cultured tissue decreased rapidly during the first 2 d despite the presence of the three hormones, but on the 3rd d tissue cultured with either insulin and prolactin or all three hormones regained the ability to synthesize milk proteins, casein, and α-lactalbumin: a greater increase occurred in the three hormone system. The delayed addition of prolactin on Day 2 to the culture system containing insulin and cortisol also stimulated casein synthesis. The addition of cytarabine, which inhibited insulin-dependent cell proliferation in cultured expiants, did not block the rebound of milk protein synthesis. These results indicate that in the presence of insulin, cortisol, and prolactin mammary epithelial cells in culture first lose and then regain the ability of synthesizing milk protein without requiring the formation of new daughter cells.
ISSN:0073-5655
2327-4328
1475-2689
DOI:10.1007/BF02633333