Involvement of a cathepsin B-like cysteine proteinase in platelet aggregation induced by tumor cells and their shed membrane vesicles

Murine 15091A mammary adenocarcinoma cells and membrane vesicles spontaneously shed from these tumor cells in culture can induce aggregation of washed human platelets. A spectrum of proteinase inhibitors was tested for their ability to inhibit 15091A induced platelet aggregation. Of the inhibitors t...

Full description

Saved in:
Bibliographic Details
Published in:Clinical & experimental metastasis 1983-10, Vol.1 (4), p.297-307
Main Authors: Cavanaugh, P G, Sloane, B F, Bajkowski, A S, Gasic, G J, Gasic, T B, Honn, K V
Format: Article
Language:English
Subjects:
Adenocarcinoma - enzymology
Adenocarcinoma - physiopathology
Adenocarcinoma - ultrastructure
Animals
Antipain - physiology
Cathepsin B
Cathepsins - analysis
Cathepsins - pharmacology
Cell Membrane - metabolism
Cell Membrane - physiology
Cell Membrane - ultrastructure
Cysteine Endopeptidases
Endopeptidases - analysis
Endopeptidases - pharmacology
In Vitro Techniques
Leupeptins - pharmacology
Mammary Neoplasms, Experimental - enzymology
Mammary Neoplasms, Experimental - physiopathology
Mammary Neoplasms, Experimental - ultrastructure
Membrane Proteins - analysis
Mice
Platelet Aggregation - drug effects
Protease Inhibitors - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Murine 15091A mammary adenocarcinoma cells and membrane vesicles spontaneously shed from these tumor cells in culture can induce aggregation of washed human platelets. A spectrum of proteinase inhibitors was tested for their ability to inhibit 15091A induced platelet aggregation. Of the inhibitors tested the most effective were those selective for cysteine proteinases. The effect of the spectrum of proteinase inhibitors on 15091A induced platelet aggregation was compared to the effect on cathepsin B-like cysteine proteinase activity in homogenates of 15091A tumor cells and their spontaneously shed vesicles. The results suggest that there is a correlation between activity of a cathepsin B-like proteinase in 15091A cells and vesicles and the ability of these cells and vesicles to induce aggregation of washed human platelets.
ISSN:0262-0898
1573-7276
DOI:10.1007/bf00121192